Antibacterial effect of silver nanoparticles on Staphylococcus aureus. 2011

Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, P.O. Box: 19835-389, Tehran, Iran. f_mirzajnai@scientist.com

Antibacterial activity of silver nanoparticles (AgNPs) was investigated using Staphylococcus aureus PTCC1431 as a model of Gram-positive bacteria. The mechanism of antibacterial activity of AgNPs was then studied by analyzing the growth, morphology, and molecular variations in the cell wall. Experimental data showed that AgNPs at a concentration of 4 μg/ml completely inhibited bacterial growth. Transmission electron microscopy results confirmed cell wall damage produced by AgNPs as well as accumulation of AgNPs in the bacterial membrane. Meanwhile, the AgNP-treated bacteria were monitored by circular dichroism to reveal peptidoglycan variations. Some degree of variation in the α-helix position of the peptide chain was observed. Moreover, increasing the AgNP concentration to 8 μg/ml resulted in release of muramic acid (MA) into the medium, which could be attributed to cell wall distraction. A gas chromatography-tandem mass spectrometry analysis and release of MA, as a bacterial indicator, showed that glycan strands may also be decomposed as a result of AgNP treatment.

UI MeSH Term Description Entries
D008826 Microbial Sensitivity Tests Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses). Bacterial Sensitivity Tests,Drug Sensitivity Assay, Microbial,Minimum Inhibitory Concentration,Antibacterial Susceptibility Breakpoint Determination,Antibiogram,Antimicrobial Susceptibility Breakpoint Determination,Bacterial Sensitivity Test,Breakpoint Determination, Antibacterial Susceptibility,Breakpoint Determination, Antimicrobial Susceptibility,Fungal Drug Sensitivity Tests,Fungus Drug Sensitivity Tests,Sensitivity Test, Bacterial,Sensitivity Tests, Bacterial,Test, Bacterial Sensitivity,Tests, Bacterial Sensitivity,Viral Drug Sensitivity Tests,Virus Drug Sensitivity Tests,Antibiograms,Concentration, Minimum Inhibitory,Concentrations, Minimum Inhibitory,Inhibitory Concentration, Minimum,Inhibitory Concentrations, Minimum,Microbial Sensitivity Test,Minimum Inhibitory Concentrations,Sensitivity Test, Microbial,Sensitivity Tests, Microbial,Test, Microbial Sensitivity,Tests, Microbial Sensitivity
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D012834 Silver An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D053768 Metal Nanoparticles Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver. Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic

Related Publications

Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
February 2011, Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
September 2021, European review for medical and pharmacological sciences,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
July 2014, Journal of nanobiotechnology,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
June 2007, Nanomedicine : nanotechnology, biology, and medicine,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
April 2011, Nanotechnology,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
January 2024, Frontiers in microbiology,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
February 2019, FEMS microbiology letters,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
October 2015, Archives of pharmacal research,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
January 2019, The Journal of antibiotics,
Fateme Mirzajani, and Alireza Ghassempour, and Atousa Aliahmadi, and Mohammad Ali Esmaeili
January 1970, Mikrobiolohichnyi zhurnal,
Copied contents to your clipboard!