-transparent.png){kind=logo}
A parathyroid hormone-related peptide (PTHRP) has been identified in human tumors associated with the syndrome of humoral hypercalcemia of malignancy. While parathyroid hormone (PTH) gene expression appears to be limited to the parathyroid glands, PTHRP mRNA has been identified in a variety of normal tissues. To investigate the apparent expression of the PTHRP in the central nervous system, we examined extracts of whole rat brain for PTHRP bioactivity by measuring adenylate cyclase-stimulating activity (ACSA) in a PTH-sensitive assay. Extracts consistently contained ACSA and this activity was completely inhibited by a PTHRP antiserum but was unaffected by a PTH antiserum. ACSA was found in a number of anatomic subregions of rat brain, being greatest in the cortex and telencephalon. RNase protection analysis revealed PTHRP transcripts in total RNA prepared from whole rat brain and from the same anatomic subregions. By in situ hybridization histochemistry, we found that the highest levels of PTHRP gene expression occurred in neurons of the cerebral cortex, hippocampus, and cerebellar cortex. These studies demonstrate that both PTHRP mRNA and biological activity are present in a number of regions of rat brain. The widespread expression of this peptide by multiple types of neurons suggests that the PTHRP may play a general role in neuronal physiology.
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
March 2010,
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
September 1987,
Science (New York, N.Y.),
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
June 1995,
Nucleic acids research,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
July 1997,
Current opinion in nephrology and hypertension,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
April 2023,
Journal of personalized medicine,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
October 1999,
The Journal of biological chemistry,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
January 2003,
In vivo (Athens, Greece),
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
January 1995,
Mineral and electrolyte metabolism,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
June 1992,
Annals of the New York Academy of Sciences,
E C Weir, and
M L Brines, and
K Ikeda, and
W J Burtis, and
A E Broadus, and
R J Robbins
January 1985,
Peptides,
