Biomaterial Database

Evaluation of the selective and differential ET medium for detection of Edwardsiella tarda in aquaculture systems. 2011

N Castro, and A E Toranzo, and S Nuñez, and B Magariños

Departamento de Microbiología y Parasitología, Facultad de Biología-CIBUS e Instituto de Acuicultura, Universidad de Santiago de Compostela, Santiago de Compostela, Spain. nuria.castro@usc.es

OBJECTIVE Edwardsiella tarda is an important pathogen in aquaculture where it can cause serious losses. A rapid detection of it is vital to minimize the mortalities caused by this disease, and in this work, the effectiveness of the selective differential Edw. tarda medium (ET) was evaluated for the diagnosis of edwardsiellosis as well as for its possible use in epidemiological studies. RESULTS ET medium was evaluated in parallel with the commercial Salmonella-Shigella agar (SS), which is usually employed for the selective isolation of enteric bacilli. Moreover, two general media (TSA-1 and MA) were employed as a control. The results obtained showed that ET is distinctly selective for the isolation of Edw. tarda, allowing its recovery from mixed cultures and natural samples as a unique species. In contrast, although colonies of Edw. tarda could be clearly distinguishable in SS because of the appearance of a characteristic black centre, other enteric and nonenteric bacterial species were also able to grow on this medium. CONCLUSIONS We recommend ET agar as an useful medium for the primary isolation of Edw. tarda from aquaculture samples. CONCLUSIONS The results obtained support ET medium as the most appropriate to develop epidemiological studies of edwardsiellosis in aquaculture and permits an earlier diagnosis of this important disease.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D004756	Enterobacteriaceae Infections	Infections with bacteria of the family ENTEROBACTERIACEAE.	Enterobacterial Infections,Cronobacter Infections,Infections, Enterobacteriaceae,Infections, Enterobacterial,Cronobacter Infection,Enterobacteriaceae Infection,Enterobacterial Infection,Infection, Cronobacter,Infection, Enterobacteriaceae,Infection, Enterobacterial,Infections, Cronobacter
D005393	Fish Diseases	Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates).	Disease, Fish,Diseases, Fish,Fish Disease
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017756	Aquaculture	The farming, breeding, rearing, and harvesting of plants and animals in all types of water environments including ponds, rivers, lakes, and the ocean.	Bioflocs,Aquacultures,Biofloc
D020609	Edwardsiella tarda	A species of EDWARDSIELLA distinguished by its hydrogen sulfide production. (From Bergey's Manual of Determinative Bacteriology, 9th ed)	Edwardsiella anguillimortifera,Paracolobactrum anguillimortiferum