Biomaterial Database

[Interactions of the phytopathogenic bacteria Erwinia with phage P1 CRL100 CML]. 1990

N A Beliasova, and V A Prokulevich, and Iu K Fomichev

The bacteriophage P1 Cm clr100 lysogenises the bacteria E. chrysanthemi, E. aroideae, E. atroseptica being localized in the cytoplasm, replicating but causing no cell lysis. The prophage induction results in transformation of the lysogenic bacteria E. chrysanthemi into nonviable filamentous cells. However, a portion of cells gets rid of the prophage and gives rise to normal heritage inheritors permitting to use the bacteriophage as an efficient vehicle for introducing the transposons into the chromosome of E. chrysanthemi. The transposon Tn9 has been found to insert into the different chromosomal sites causing no inactivation of the genes, while the transposition of Tn5 from the bacteriophage P1::Tn5Cmclr100 induces the different mutations with the frequency up to 3%. The bacteriophage P1Cmclr100 may also serve a tool for construction of the homology regions in the chromosome of E. chrysanthemi and Flac+ plasmid for further construction of Hfr-type donors.

UI	MeSH Term	Description	Entries
D008242	Lysogeny	The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.	Integration, Prophage,Prophage Integration,Integrations, Prophage,Prophage Integrations
D004251	DNA Transposable Elements	Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.	DNA Insertion Elements,DNA Transposons,IS Elements,Insertion Sequence Elements,Tn Elements,Transposable Elements,Elements, Insertion Sequence,Sequence Elements, Insertion,DNA Insertion Element,DNA Transposable Element,DNA Transposon,Element, DNA Insertion,Element, DNA Transposable,Element, IS,Element, Insertion Sequence,Element, Tn,Element, Transposable,Elements, DNA Insertion,Elements, DNA Transposable,Elements, IS,Elements, Tn,Elements, Transposable,IS Element,Insertion Element, DNA,Insertion Elements, DNA,Insertion Sequence Element,Sequence Element, Insertion,Tn Element,Transposable Element,Transposable Element, DNA,Transposable Elements, DNA,Transposon, DNA,Transposons, DNA
D004885	Erwinia	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria whose organisms are associated with plants as pathogens, saprophytes, or as constituents of the epiphytic flora.
D005822	Genetic Vectors	DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.	Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D001435	Bacteriophages	Viruses whose hosts are bacterial cells.	Phages,Bacteriophage,Phage