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Human plasma fibronectin contains a latent proteinase that after activation cleaves gelatin and fibronectin. The autoactivation propensity of the two purified cathepsin D-produced fragments of fibronectin (190 and 120 kDa) was compared. Both polypeptides were spontaneously activated in the presence of Ca2+. This activation was inhibited by EDTA. The active gelatinase was isolated from the autodigest of the 190-kDa fragment. Among various protein substrates, including laminin and native type I and IV collagens, the purified enzyme degraded only gelatin and fibronectin. We have named this proteinase FN-gelatinase. FN-gelatinase is inhibited by phenylmethanesulfonyl fluoride and also by pepstatin A like retroviral aspartic proteinases. The amino-acid composition of the purified enzyme (35 kDa) was compared with the entire fibronectin sequence using the computer programme FIT. The optimal fit indicated that the 35-kDa fragment corresponds to the stretch # 1043-1404. This sequence contains a 93-residue segment (# 1140-1233) analogous to retroviral aspartic proteinases, comprising the sequence DTG of their putative active site.
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
August 1986,
Proceedings of the National Academy of Sciences of the United States of America,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
May 1999,
Journal of protein chemistry,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
September 1979,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
April 1986,
The Journal of biological chemistry,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
November 2007,
Annals of the rheumatic diseases,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
December 1982,
Journal of immunological methods,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
January 1986,
European journal of biochemistry,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
January 1972,
Medical microbiology and immunology,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
June 1948,
The Health center journal,
T Planchenault, and
S Lambert Vidmar, and
J M Imhoff, and
X Blondeau, and
I Emod, and
F Lottspeich, and
V Keil-Dlouha
January 1983,
Invasion & metastasis,
