BIOMAT Database Logo BIOMAT Database Logo

Production and characterization of a humanized single-chain antibody against human integrin alphav beta3 protein. 2011

Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.

Anti-angiogenesis therapy is an emerging strategy for cancer treatment. This therapy has many advantages over existing treatments, such as fewer side effects, fewer resistance problems, and a broader tumor type spectrum. Integrin αvβ3 is a heterodimeric transmembrane glycoprotein that has been demonstrated to play a key role in tumor angiogenesis and metastasis. We have used a phage antibody display to humanize a mouse monoclonal antibody (mAb E10) against human integrin αvβ3 with a predetermined CDR3 gene. Three human phage antibodies were developed. Analysis of the humanized phage antibodies by phage ELISA revealed that the antibodies retained high antigen-binding activity and detected the same epitope as the parent mAb E10. A humanized single chain Fv (scFv) antibody was expressed in Escherichia coli in a soluble form. Analysis of the purified scFv indicated that it has the same specificity and affinity as the original mAb. Cell viability assays and xenograft model results suggested that the humanized scFv possesses anti-tumor growth activity in vitro and in vivo. This successful production of a humanized scFv with the ability to inhibit αvβ3-mediated cancer cell growth may provide a novel candidate for integrin αvβ3-targeted therapy.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D057127 Single-Chain Antibodies A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use. Fv Antibody Fragments, Single-Chain,ScFv Antibodies,Single-Chain Fv,Single-Chain Fv Antibody,Single-Chain Fv Antibody Fragments,Single-Chain Variable Fragment,Single-Chain Variable Fragment Antibodies,Single-Chain Variable Fragment Antibody,Single-Chain Variable Fragments,Antibodies, ScFv,Antibodies, Single-Chain,Antibody, Single-Chain Fv,Fragment, Single-Chain Variable,Fragments, Single-Chain Variable,Fv Antibody Fragments, Single Chain,Fv Antibody, Single-Chain,Fv, Single-Chain,Single Chain Antibodies,Single Chain Fv,Single Chain Fv Antibody,Single Chain Fv Antibody Fragments,Single Chain Variable Fragment,Single Chain Variable Fragment Antibodies,Single Chain Variable Fragment Antibody,Single Chain Variable Fragments,Variable Fragment, Single-Chain,Variable Fragments, Single-Chain
D058846 Antibodies, Monoclonal, Murine-Derived Antibodies obtained from a single clone of cells grown in mice or rats. Murine-Derived Monoclonal Antibodies,Antibodies, Murine-Derived Monoclonal,Monoclonal Antibodies, Murine-Derived,Murine Derived Monoclonal Antibodies
D022801 Complementarity Determining Regions Three regions (CDR1; CDR2 and CDR3) of amino acid sequence in the IMMUNOGLOBULIN VARIABLE REGION that are highly divergent. Together the CDRs from the light and heavy immunoglobulin chains form a surface that is complementary to the antigen. These regions are also present in other members of the immunoglobulin superfamily, for example, T-cell receptors (RECEPTORS, ANTIGEN, T-CELL). Complementarity Determining Region,Complementarity Determining Region 1,Complementarity Determining Region 2,Complementarity Determining Region 3,Complementarity Determining Region I,Complementarity Determining Region II,Complementarity Determining Region III,Complementarity-Determining Region,Complementarity-Determining Region 3,Hypervariable Region, Immunoglobulin,Hypervariable Regions, Immunoglobulin,Third Complementarity-Determining Region,Complementarity-Determining Region 3s,Complementarity-Determining Region, Third,Complementarity-Determining Regions,Complementarity-Determining Regions, Third,Immunoglobulin Hypervariable Region,Immunoglobulin Hypervariable Regions,Region, Complementarity Determining,Region, Immunoglobulin Hypervariable,Regions, Complementarity Determining,Regions, Complementarity-Determining,Regions, Immunoglobulin Hypervariable,Third Complementarity Determining Region,Third Complementarity-Determining Regions
D039302 Integrin alphaVbeta3 An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN). Integrin alpha-v beta-3,Receptors, alpha-v beta-3 Integrin,Vitronectin Receptor,alpha-v beta-3 Integrin Receptors,Integrin alpha v beta 3,Receptor, Vitronectin,Receptors, alpha v beta 3 Integrin,alpha v beta 3 Integrin Receptors,alpha-v beta-3, Integrin,alphaVbeta3, Integrin,beta-3, Integrin alpha-v

Related Publications

Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
A humanized single-chain variable fragment antibody against beta3 integrin in Escherichia coli.
December 2011, Hybridoma (2005),
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Large-scale production, bacterial localization assessment and immobilized metal affinity chromatography purification of a human single-chain Fv antibody against alphaIIb-beta3 integrin.
August 2006, International journal of biological macromolecules,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Expression of integrin subunits alphav and beta3 in acute lung inflammation.
May 2004, Histochemistry and cell biology,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Inducible tyrosine phosphorylation of the beta3 integrin requires the alphaV integrin cytoplasmic tail.
December 1996, The Journal of biological chemistry,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Rapid purification of a new humanized single-chain Fv antibody/human interleukin-2 fusion protein reactive against HER2 receptor.
October 2004, Acta biochimica et biophysica Sinica,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
The zebrafish vitronectin receptor: characterization of integrin alphaV and beta3 expression patterns in early vertebrate development.
August 2007, Developmental dynamics : an official publication of the American Association of Anatomists,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Production of a human single-chain variable fragment antibody against esophageal carcinoma.
September 2004, World journal of gastroenterology,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Production and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein.
April 2010, Applied microbiology and biotechnology,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Characterization of an intracellular humanized single-chain antibody to matrix protein (M1) of H5N1 virus.
January 2022, PloS one,
Dabin Liu, and Chen Wang, and Cun Li, and Xin Zhang, and Baozhong Zhang, and Zhiqiang Mi, and Xiaoping An, and Yigang Tong
Platelet fragmentation requires a specific structural conformation of human monoclonal antibody against beta3 integrin.
February 2008, The Journal of biological chemistry,
Copied contents to your clipboard!