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Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
March 1990,
FEBS letters,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
January 1986,
Methods in enzymology,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
November 1984,
The Biochemical journal,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
April 1988,
Journal of biochemistry,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
April 1979,
Biochemical and biophysical research communications,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
October 1995,
Protein expression and purification,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
November 1990,
Biochimica et biophysica acta,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
November 1984,
Biochimica et biophysica acta,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
November 1982,
The Journal of biological chemistry,
W Z Gai, and
S M Sun, and
N Sone, and
S H Chan
June 1984,
Biochemistry,
