The earliest intrathymic precursors of CD8α(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells. 2011

Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.

The dendritic cells (DCs) present in lymphoid and non-lymphoid organs are generated from progenitors with myeloid-restricted potential. However, in the thymus a major subset of DCs expressing CD8α and langerin (CD207) appears to stand apart from all other DCs in that it is thought to derive from progenitors with lymphoid potential. Using mice expressing a fluorescent reporter and a diphtheria toxin receptor under the control of the cd207 gene, we demonstrated that CD207(+) CD8α(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors that express markers that are normally present on all (CD11c(+) and MHCII molecules) or on some (CD207, CD135, CD8α, CX3CR1) DC subsets. Those intrathymic myeloid-type precursors correspond to CD44(+) CD25(-) double-negative 1c (DN1c) cells and are continuously renewed from bone marrow-derived canonical DC precursors. In conclusion, our results demonstrate that the earliest intrathymic precursors of CD8α(+) thymic DCs correspond to myeloid-type DN1c cells and support the view that under physiological conditions myeloid-restricted progenitors generate the whole constellation of DCs present in the body including the thymus.

UI MeSH Term Description Entries
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003713 Dendritic Cells Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION). Dendritic Cells, Interdigitating,Interdigitating Cells,Plasmacytoid Dendritic Cells,Veiled Cells,Dendritic Cells, Interstitial,Dendritic Cells, Plasmacytoid,Interdigitating Dendritic Cells,Interstitial Dendritic Cells,Cell, Dendritic,Cell, Interdigitating,Cell, Interdigitating Dendritic,Cell, Interstitial Dendritic,Cell, Plasmacytoid Dendritic,Cell, Veiled,Cells, Dendritic,Cells, Interdigitating,Cells, Interdigitating Dendritic,Cells, Interstitial Dendritic,Cells, Plasmacytoid Dendritic,Cells, Veiled,Dendritic Cell,Dendritic Cell, Interdigitating,Dendritic Cell, Interstitial,Dendritic Cell, Plasmacytoid,Interdigitating Cell,Interdigitating Dendritic Cell,Interstitial Dendritic Cell,Plasmacytoid Dendritic Cell,Veiled Cell
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000954 Antigens, Surface Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated. Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell
D013154 Spleen An encapsulated lymphatic organ through which venous blood filters.

Related Publications

Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
September 2011, Blood,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
December 2016, Journal of autoimmunity,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
February 1996, Journal of immunology (Baltimore, Md. : 1950),
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
January 2001, Methods in molecular medicine,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
September 2012, Molecules and cells,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
April 2008, Nature,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
January 2012, Development (Cambridge, England),
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
July 2008, Journal of visualized experiments : JoVE,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
February 2001, International reviews of immunology,
Hervé Luche, and Laurence Ardouin, and Pearline Teo, and Peter See, and Sandrine Henri, and Miriam Merad, and Florent Ginhoux, and Bernard Malissen
January 2010, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!