-transparent.png){kind=logo}
Conformational alterations induced by streptomycin in the bacterial ribosome have been investigated using as probes, ethidium bromide, N-[14C]ethylmaleimide and a spin label nitroxide analog of N-ethylmaleimide. 1. The binding of the antibiotic to the ribosome does not affect the reactivity of sulfhydryl groups towards N-ethylmaleimide. 2. The motional freedom of spin labels bound to ribosomal proteins S1 and S18 is increased but it is hardly affected at other labeled sites. This observation suggests that the binding of streptomycin causes a local loosening of the ribosomal structure. 3. Ribosomes are found to bind less ethidium bromide in the presence of streptomycin, which suggests that the binding of streptomycin decreases the degree of organization of ribosomal RNA.
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
August 1973,
Journal of molecular biology,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
December 2000,
Journal of molecular biology,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
January 1969,
Cold Spring Harbor symposia on quantitative biology,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
November 1973,
FEBS letters,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
August 1979,
The Journal of biological chemistry,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
November 2002,
Biochemical Society transactions,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
March 1978,
FEBS letters,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
August 1979,
The Journal of biological chemistry,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
October 1974,
Biochemical and biophysical research communications,
L Brakier-Gingras, and
G Boileau, and
S Glorieux, and
N Brisson
January 1984,
The International journal of biochemistry,
