Osteocalcin and N-telopeptides of type I collagen marker levels in gingival crevicular fluid during different stages of orthodontic tooth movement. 2011

Sarah A Alfaqeeh, and Sukumaran Anil
Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

BACKGROUND The aim of the study was to quantitatively and qualitatively analyze changes in the gingival crevicular fluid (GCF) during orthodontic movement by assessing the levels of cross-linked N-telopeptides of type I collagen marker (NTx) and osteocalcin (OC) activity. METHODS Twenty patients requiring all first premolars to be extracted were selected and treated with conventional straight-wire mechanotherapy. The canines were retracted with closed-coil springs. The maxillary canine on one side acted as the experimental site, and the contralateral canine was the control. GCF was collected from around the canines before retraction, and 1 hour, 1 day, 7 days, 14 days, and 21 days after retraction. GCF NTx and OC levels were estimated and compared with the control side. RESULTS The results showed statistically significant changes in NTx and OC levels on days 7, 14, and 21 when we compared the experimental and control sides. The peak in all activity of the variables occurred on day 14 after retraction. CONCLUSIONS The study showed that NTx and OC levels can be successfully estimated in the GCF, and its increased levels might indicate the active tooth movement phase in orthodontic therapy.

UI MeSH Term Description Entries
D008297 Male Males
D008311 Malocclusion, Angle Class I Malocclusion in which the mandible and maxilla are anteroposteriorly normal as reflected by the relationship of the first permanent molar (i.e., in neutroclusion), but in which individual teeth are abnormally related to each other. Angle Class I,Class I, Angle
D008437 Maxilla One of a pair of irregularly shaped bones that form the upper jaw. A maxillary bone provides tooth sockets for the superior teeth, forms part of the ORBIT, and contains the MAXILLARY SINUS. Maxillae,Maxillary Bone,Bone, Maxillary,Bones, Maxillary,Maxillary Bones,Maxillas
D009969 Orthodontic Wires Wires of various dimensions and grades made of stainless steel or precious metal. They are used in orthodontic treatment. Orthodontic Wire,Wire, Orthodontic,Wires, Orthodontic
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D003481 Cuspid The third tooth to the left and to the right of the midline of either jaw, situated between the second INCISOR and the premolar teeth (BICUSPID). (Jablonski, Dictionary of Dentistry, 1992, p817) Canine Tooth,Canine Teeth,Cuspids,Teeth, Canine,Tooth, Canine
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D005500 Follow-Up Studies Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease. Followup Studies,Follow Up Studies,Follow-Up Study,Followup Study,Studies, Follow-Up,Studies, Followup,Study, Follow-Up,Study, Followup
D005883 Gingival Crevicular Fluid A fluid occurring in minute amounts in the gingival crevice, believed by some authorities to be an inflammatory exudate and by others to cleanse material from the crevice, containing sticky plasma proteins which improve adhesions of the epithelial attachment, have antimicrobial properties, and exert antibody activity. (From Jablonski, Illustrated Dictionary of Dentistry, 1982) Gingival Exudate,Crevicular Fluid, Gingival,Crevicular Fluids, Gingival,Exudate, Gingival,Exudates, Gingival,Fluid, Gingival Crevicular,Fluids, Gingival Crevicular,Gingival Crevicular Fluids,Gingival Exudates

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