Biomaterial Database

Susceptibility of Aedes albopictus to infection with eastern equine encephalomyelitis virus. 1990

T W Scott, and L H Lorenz, and S C Weaver

Department of Entomology, University of Maryland, College Park 20742.

We examined susceptibility of a strain of Aedes albopictus from Houston, Texas to experimental infection with eastern equine encephalomyelitis (EEE) virus. After 15 days of extrinsic incubation, all Ae. albopictus examined by the cell culture assay and fluorescent antibody staining were infected but only 57% (4/7) of the mosquitoes that refed transmitted virus by bite. Data supported the concept of a salivary gland infection barrier to EEE virus in Ae. albopictus and the conclusion that virus replicates in fat body following dissemination from the midgut and prior to infection of salivary glands. Assay of adult progeny from females that fed on viremic chicks and fluorescent antibody studies of infected females failed to provide evidence that EEE virus is transmitted vertically by Ae. albopictus.

UI	MeSH Term	Description	Entries
D007303	Insect Vectors	Insects that transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.	Insect Vector,Vector, Insect,Vectors, Insect
D008297	Male		Males
D004663	Encephalitis Virus, Eastern Equine	A species of ALPHAVIRUS causing encephalomyelitis in Equidae and humans. The virus ranges along the Atlantic seaboard of the United States and Canada and as far south as the Caribbean, Mexico, and parts of Central and South America. Infections in horses show a mortality of up to 90 percent and in humans as high as 80 percent in epidemics.	EEE Virus,Eastern Equine Encephalomyelitis Virus,Eastern equine encephalitis virus,Encephalomyelitis Virus, Eastern Equine,EEE Viruses
D005260	Female		Females
D005455	Fluorescent Antibody Technique	Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.	Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000330	Aedes	A genus of mosquitoes (CULICIDAE) frequently found in tropical and subtropical regions. YELLOW FEVER and DENGUE are two of the diseases that can be transmitted by species of this genus.	Aede
D000524	Alphavirus	A genus of TOGAVIRIDAE, also known as Group A arboviruses. They are serologically related to each other and are transmitted by mosquitoes. The type species is the SINDBIS VIRUS.	Arboviruses, Group A,Barmah forest virus,Getah virus,Sagiyama virus,Alpha Virus,Alphaviruses,Alpha Viruses,Arbovirus, Group A,Group A Arbovirus,Group A Arboviruses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral