NAADP mediates ATP-induced Ca2+ signals in astrocytes. 2011

Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
Institut de Neurociències and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.

Intracellular Ca(2+) signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca(2+) in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca(2+) responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.

UI MeSH Term Description Entries
D009249 NADP Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed) Coenzyme II,Nicotinamide-Adenine Dinucleotide Phosphate,Triphosphopyridine Nucleotide,NADPH,Dinucleotide Phosphate, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide Phosphate,Nucleotide, Triphosphopyridine,Phosphate, Nicotinamide-Adenine Dinucleotide
D001920 Bradykinin A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg,Bradykinin Acetate, (9-D-Arg)-Isomer,Bradykinin Diacetate,Bradykinin Hydrochloride,Bradykinin Triacetate,Bradykinin, (1-D-Arg)-Isomer,Bradykinin, (2-D-Pro)-Isomer,Bradykinin, (2-D-Pro-3-D-Pro-7-D-Pro)-Isomer,Bradykinin, (2-D-Pro-7-D-Pro)-Isomer,Bradykinin, (3-D-Pro)-Isomer,Bradykinin, (3-D-Pro-7-D-Pro)-Isomer,Bradykinin, (5-D-Phe)-Isomer,Bradykinin, (5-D-Phe-8-D-Phe)-Isomer,Bradykinin, (6-D-Ser)-Isomer,Bradykinin, (7-D-Pro)-Isomer,Bradykinin, (8-D-Phe)-Isomer,Bradykinin, (9-D-Arg)-Isomer,Arg Pro Pro Gly Phe Ser Pro Phe Arg
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002614 Chelating Agents Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS. Chelating Agent,Chelator,Complexons,Metal Antagonists,Chelators,Metal Chelating Agents,Agent, Chelating,Agents, Chelating,Agents, Metal Chelating,Antagonists, Metal,Chelating Agents, Metal
D004533 Egtazic Acid A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID. EGTA,Ethylene Glycol Tetraacetic Acid,EGATA,Egtazic Acid Disodium Salt,Egtazic Acid Potassium Salt,Egtazic Acid Sodium Salt,Ethylene Glycol Bis(2-aminoethyl ether)tetraacetic Acid,Ethylenebis(oxyethylenenitrile)tetraacetic Acid,GEDTA,Glycoletherdiamine-N,N,N',N'-tetraacetic Acid,Magnesium-EGTA,Tetrasodium EGTA,Acid, Egtazic,EGTA, Tetrasodium,Magnesium EGTA
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001253 Astrocytes A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury. Astroglia,Astroglia Cells,Astroglial Cells,Astrocyte,Astroglia Cell,Astroglial Cell,Astroglias,Cell, Astroglia,Cell, Astroglial
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

Related Publications

Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
September 2006, Biochemical and biophysical research communications,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
June 1998, Pflugers Archiv : European journal of physiology,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
May 2018, The Journal of physiology,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
May 2021, Scientific reports,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
January 2018, Frontiers in molecular neuroscience,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
May 1994, Pflugers Archiv : European journal of physiology,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
February 2004, Biology of the cell,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
January 2004, Journal of neurochemistry,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
September 2017, Journal of neurochemistry,
Miquel Barceló-Torns, and Alexander M Lewis, and Albert Gubern, and David Barneda, and Duncan Bloor-Young, and Fernado Picatoste, and Grant C Churchill, and Enrique Claro, and Roser Masgrau
January 2023, Handbook of experimental pharmacology,
Copied contents to your clipboard!