Pharmacokinetics of ergosterol in rats using rapid resolution liquid chromatography-atmospheric pressure chemical ionization multi-stage tandem mass spectrometry and rapid resolution liquid chromatography/tandem mass spectrometry. 2011

Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
Biomedicine Key Laboratory of Shaanxi Province, The School of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China. zyy@nwu.edu.cn

Rapid resolution liquid chromatography/tandem multi-stage mass spectrometry (RRLC-MS(n)) and rapid resolution liquid chromatography/tandem mass spectrometry (RRLC/MS/MS) methods were developed for the identification and quantification of ergosterol and its metabolites from rat plasma, urine and faeces. Two metabolites (ERG1 and ERG2) were identified by RRLC/MS(n). The concentrations of the ergosterol were determined by RRLC/MS/MS. The separation was performed on an Agilent Zorbax SB-C18 with the mobile phase consisting of methanol and water (containing 0.1% formic acid). The detection was carried out by means of atmospheric pressure chemical ionization mass spectrometry in positive ion mode with multiple reaction monitoring (MRM). Linear calibration curves were obtained in the concentration range of 7-2000, 6-2000 and 8-7500 ng/mL for plasma, urine and faecal homogenate, respectively. The intra- and inter-day precision values (RSD) were below 10%. The method was applied to the pharmacokinetic properties and elimination pathway of ergosterol in rats.

UI MeSH Term Description Entries
D008297 Male Males
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D004355 Drug Stability The chemical and physical integrity of a pharmaceutical product. Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D004875 Ergosterol A steroid occurring in FUNGI. Irradiation with ULTRAVIOLET RAYS results in formation of ERGOCALCIFEROL (vitamin D2). Lumisterol,Pro-Vitamin D2,Provitamin D 2,D2, Pro-Vitamin,Pro Vitamin D2
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016018 Least-Squares Analysis A principle of estimation in which the estimates of a set of parameters in a statistical model are those quantities minimizing the sum of squared differences between the observed values of a dependent variable and the values predicted by the model. Rietveld Refinement,Analysis, Least-Squares,Least Squares,Analyses, Least-Squares,Analysis, Least Squares,Least Squares Analysis,Least-Squares Analyses,Refinement, Rietveld
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
January 1995, Rapid communications in mass spectrometry : RCM,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
July 2006, Lab on a chip,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
August 2005, Analytical and bioanalytical chemistry,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
March 1992, Journal of chromatography,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
August 1999, Journal of environmental monitoring : JEM,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
October 2008, Yao xue xue bao = Acta pharmaceutica Sinica,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
November 1999, Journal of the American Society for Mass Spectrometry,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
January 2000, Rapid communications in mass spectrometry : RCM,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
June 2010, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Ying-Yong Zhao, and Xian-Long Cheng, and Rui Liu, and Charlene C Ho, and Feng Wei, and Sui-Hong Yan, and Rui-Chao Lin, and Yongmin Zhang, and Wen-Ji Sun
January 2007, Phytochemical analysis : PCA,
Copied contents to your clipboard!