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Cyclic nucleotide phosphodiesterase activity in rat heart microsomes is attributable to several isoenzymatic forms: a cyclic AMP-specific, a cyclic GMP-specific, and a cyclic GMP-stimulated enzyme. Incubation of microsomes with an exogenous phospholipase C (C. welchii) induced a marked stimulation (+126%) of cyclic AMP phosphodiesterase and a moderate stimulation (+49%) of cyclic GMP-phosphodiesterase in the membrane-bound fraction. Besides, a notable fraction of activity was solubilized by the treatment. A parallel decrease in the activating effect of cyclic GMP on the hydrolysis of cyclic AMP was observed in the membranes (down to 18% of the control effect). It resulted from a marked stimulation of the basal activity, while the activated level was unaffected. The treatment by an exogenous phospholipase D induced more moderate modifications. The addition to microsomes of oleyl,acetyl-glycerol, but not of long chain-diacylglycerols, partly reproduced the phospholipase C effect. Phosphatidate also induced variations in phosphodiesterase activity, and could thus participate in the phospholipase effects. These results suggest that endogenous phospholipases, the activity of which is modulated by hormonal stimuli, might influence phosphodiesterase activity in cardiac membranes by producing phospholipid metabolites, with potential consequences on heart contractility.
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
August 1983,
Archives of biochemistry and biophysics,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
March 1976,
Nature,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
August 1971,
Metabolism: clinical and experimental,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
March 1974,
Life sciences,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
October 1981,
The Biochemical journal,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
March 1981,
Canadian journal of physiology and pharmacology,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
April 1982,
Molecular and cellular biochemistry,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
January 1981,
Advances in cyclic nucleotide research,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
September 1973,
Plant physiology,
M Dubois, and
G Némoz, and
M Lagarde, and
A F Prigent
January 1976,
Recent advances in studies on cardiac structure and metabolism,
