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Validation of an enzyme immuno-assay for detection of antibodies against bovine leucosis virus is described in this paper. Internal standardisation of the test was done by means of a negative control serum. With absolute extinction of the negative control serum between 100 and 200 mE, a serum sample is rated positive, if its extinction is 1.5 times above the control. The methodological sensitivity of the enzyme immuno-assay described has proved to be four times as high as that of the immunodiffusion test. The results recorded at five diagnostic laboratories suggested a sensitivity of the test of 97.6 percent (92.1 to 100 percent) and a specificity of 98.1 percent (94.4 to 100 percent). The high efficiency of the test can be confirmed by immunoblotting.
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
March 1984,
Archiv fur experimentelle Veterinarmedizin,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
September 1987,
Archiv fur experimentelle Veterinarmedizin,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
January 1978,
Annales de recherches veterinaires. Annals of veterinary research,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
August 1980,
The Veterinary record,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
January 1981,
The veterinary quarterly,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
January 1981,
The veterinary quarterly,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
January 1985,
Avian diseases,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
September 1987,
Archiv fur experimentelle Veterinarmedizin,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
February 1991,
Tierarztliche Praxis,
A F Olechnowitz, and
A Miko, and
H Koepernik, and
E Starick, and
I Fröbe
February 1987,
Journal of virological methods,
