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When isolated rat pars intermedia cells were incubated for 10 min with radioactive amino acids, one major labeled protein with a molecular weight of 30,000 +/- 1500 was extracted. This protein was shown to contain in its sequence the antigenic determinants for corticotropin and beta-melanotropin by immunoprecipitation. When the radioactivity incorporated into this large molecular weight protein during the first 10 min was chased by a further incubation in presence of an excess of unlabeled amino acid, the initial protein was degraded into several smaller peptides including beta-endorphin and beta-lipotropin. Another 18,000-dalton peptide was also observed and was tentatively identified as a large molecular form of corticotropin. From the kinetics of the maturation of the initial precursor, it is concluded that the initial cleavage of the 30,000-dalton peptide gives rise to beta-lipotropin and the 18,000-dalton form of corticotropin. beta-Lipotropin is subsequently cleaved to form beta-endorphin.
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
December 1978,
Life sciences,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
October 1977,
Proceedings of the National Academy of Sciences of the United States of America,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
October 1984,
FEBS letters,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
September 1980,
Biochemical and biophysical research communications,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
January 1979,
Annales de la nutrition et de l'alimentation,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
April 1981,
Biochemistry,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
April 1980,
Proceedings of the National Academy of Sciences of the United States of America,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
December 1979,
The Journal of biological chemistry,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
December 1984,
Biochemical Society transactions,
P Crine, and
C Gianoulakis, and
N G Seidah, and
F Gossard, and
P D Pezalla, and
M Lis, and
M Chrétien
April 1981,
Brain research,
