BIOMAT Database Logo BIOMAT Database Logo

Human thyroid cells transfected with SV40 DNA retain the TSH receptor. 1990

M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
C. H. Best Institute, University of Toronto, Ontario, Canada.

Human thyroid cells were transfected with SV40 DNA using the calcium phosphate co-precipitation technique. The transfected cells grew rapidly and could be passaged readily. TSH and the immunoglobulin fraction from a patient with hyperthyroid Graves' disease stimulated adenylate cyclase activity in the transfected cells. These cells could potentially be used in clinical assays of patients' immunoglobulins without concern for species incompatibilities. On immunoblot analysis, extracts from SV40-transfected human thyroid cells, from sheep thyroid cells and from rat FRTL5 cells reacted with the immunoglobulin fraction from the patient with hyperthyroid Graves' disease but not with the immunoglobulin fraction from a pool of serum from normal subjects. Proteins of molecular weights 77,000, 70,000 and 54,000 were identified by the patient's immunoglobulin fraction in the three thyroid-derived cell types.

UI MeSH Term Description Entries
D011989 Receptors, Thyrotropin Cell surface proteins that bind pituitary THYROTROPIN (also named thyroid stimulating hormone or TSH) and trigger intracellular changes of the target cells. TSH receptors are present in the nervous system and on target cells in the thyroid gland. Autoantibodies to TSH receptors are implicated in thyroid diseases such as GRAVES DISEASE and Hashimoto disease (THYROIDITIS, AUTOIMMUNE). Receptors, Thyroid Stimulating Hormone,TSH Receptors,Thyroid Stimulating Hormone Receptors,Thyrotropin Receptors,LATS Receptors,Receptor, LATS Immunoglobulins,Receptors, LATS,Receptors, Long-Acting Thyroid Stimulator,Receptors, TSH,TSH Receptor,Thyroid Stimulating Hormone Receptor,Thyrotropin Receptor,Receptor, TSH,Receptor, Thyrotropin,Receptors, Long Acting Thyroid Stimulator
D002472 Cell Transformation, Viral An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus. Transformation, Viral Cell,Viral Cell Transformation,Cell Transformations, Viral,Transformations, Viral Cell,Viral Cell Transformations
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005814 Genes, Viral The functional hereditary units of VIRUSES. Viral Genes,Gene, Viral,Viral Gene
D006111 Graves Disease A common form of hyperthyroidism with a diffuse hyperplastic GOITER. It is an autoimmune disorder that produces antibodies against the THYROID STIMULATING HORMONE RECEPTOR. These autoantibodies activate the TSH receptor, thereby stimulating the THYROID GLAND and hypersecretion of THYROID HORMONES. These autoantibodies can also affect the eyes (GRAVES OPHTHALMOPATHY) and the skin (Graves dermopathy). Basedow's Disease,Exophthalmic Goiter,Goiter, Exophthalmic,Graves' Disease,Basedow Disease,Hyperthyroidism, Autoimmune,Basedows Disease,Disease, Basedow,Disease, Basedow's,Disease, Graves,Disease, Graves',Exophthalmic Goiters,Goiters, Exophthalmic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D013539 Simian virus 40 A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures. SV40 Virus,Vacuolating Agent,Polyomavirus macacae,SV 40 Virus,SV 40 Viruses,SV40 Viruses,Vacuolating Agents
D013961 Thyroid Gland A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively. Thyroid,Gland, Thyroid,Glands, Thyroid,Thyroid Glands,Thyroids

Related Publications

M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Malignant transformation of rat thyroid cells transfected with the human TSH receptor cDNA.
February 1992, Biochemical and biophysical research communications,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Differential effect of thyroid-stimulating hormone (TSH) on intracellular free calcium and cAMP in cells transfected with the human TSH receptor.
June 1998, The Journal of endocrinology,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Thyrotropin (TSH)-induced receptor internalization in nonthyroidal cells transfected with a human TSH-receptor complementary deoxyribonucleic acid.
May 1994, Endocrinology,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
An investigation of the ability of TSH and Graves' immunoglobulin G to increase intracellular calcium in human thyroid cells, rat FRTL-5 thyroid cells and eukaryotic cells transfected with the human TSH receptor.
December 1994, The Journal of endocrinology,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Detection of antibodies blocking thyrotropin effect using Chinese hamster ovary cells transfected with the cloned human TSH receptor.
November 1994, Journal of endocrinological investigation,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Measurement of thyroid stimulating immunoglobulins in a new cell line transfected with a functional human TSH receptor (JPO9 cells), compared with an assay using FRTL-5 cells.
May 1994, Clinical endocrinology,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
One monoclonal antibody to human thyrotropin (TSH) receptor agonist of TSH hormone stimulates the proliferation of human thyroid cells.
April 1995, Cellular immunology,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Extended life of human corneal endothelial cells transfected with the SV40 large T antigen.
May 1993, Investigative ophthalmology & visual science,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Calcium influx induced by activation of receptor tyrosine kinases in SV40-transfected human corneal endothelial cells.
October 2003, Experimental eye research,
M C Eggo, and J E Errick, and M A Pratt, and G N Burrow
Alterations of DNA content in human endometrial stromal cells transfected with a temperature-sensitive SV40: tetraploidization and physiological consequences.
January 1992, Carcinogenesis,
Copied contents to your clipboard!