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A refined complement-enhanced neutralization test for detecting antibodies to Junin virus. 1990

J G Barrera Oro, and K T McKee, and J Spisso, and B G Mahlandt, and J I Maiztegui
Salk Institute, Government Services Division, Swiftwater, Pennsylvania.

A refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against Junin (Argentine hemorrhagic fever) virus. The assay measured neutralizing antibodies after natural as well as vaccine-induced Junin virus infections. Among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. Enhanced sensitivity was achieved by using a standardized complement source (vs human or animal serum) for virus dilution, incubation of virus-serum mixtures at 36 degrees C for 2 h (vs overnight at 4 degrees C) prior to plaque assay, control of age and density of cell monolayers, and variation in overlay conditions.

UI MeSH Term Description Entries
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D006477 Arenaviruses, New World One of two groups of viruses in the ARENAVIRUS genus and considered part of the New World complex. It includes JUNIN VIRUS; PICHINDE VIRUS; Amapari virus, and Machupo virus among others. They are the cause of human hemorrhagic fevers mostly in Central and South America. Amapari virus,Chapare mammarenavirus,Hemorrhagic Fever Virus, Bolivian,Hemorrhagic Fever Viruses, American,Machupo virus,New World Arenaviruses,Sabia virus,Tacaribe virus,Allpahuayo virus,American Hemorrhagic Fever Virus,Chapare virus,Guanarito virus,Pirital virus,Tacaribe Complex Viruses,Allpahuayo viruses,Chapare mammarenaviruses,Chapare viruses,Guanarito viruses,Pirital viruses,Tacaribe viruses,viruses, Tacaribe
D006478 Hemorrhagic Fever, American Diseases caused by American hemorrhagic fever viruses (ARENAVIRUSES, NEW WORLD). American Hemorrhagic Fever,Argentine Hemorrhagic Fever,Bolivian Hemorrhagic Fever,Brazilian Hemorrhagic Fever,Hemorrhagic Fever, Argentinian,Hemorrhagic Fever, Bolivian,Hemorrhagic Fever, Brazilian,Junin virus Infection,Machupo virus Infection,Sabia virus Infection,American Hemorrhagic Fevers,Argentinian Hemorrhagic Fever,Bolivian Hemorrhagic Fevers,Brazilian Hemorrhagic Fevers,Fever, American Hemorrhagic,Fever, Argentine Hemorrhagic,Fever, Argentinian Hemorrhagic,Fever, Bolivian Hemorrhagic,Fever, Brazilian Hemorrhagic,Hemorrhagic Fever, Argentine,Infection, Junin virus,Infection, Machupo virus,Infection, Sabia virus,Junin virus Infections,Machupo virus Infections,Sabia virus Infections
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000704 Analysis of Variance A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable. ANOVA,Analysis, Variance,Variance Analysis,Analyses, Variance,Variance Analyses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell
D014765 Viral Vaccines Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease. Viral Vaccine,Vaccine, Viral,Vaccines, Viral

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