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The effect of glucocorticoids on the atrial natriuretic factor (ANF)-mediated formation of cyclic guanosine monophosphate (cGMP) by intact vascular smooth muscle cells (VSMC) was studied in rats. Cultured VSMC were obtained from the renal arteries of 14-week-old Wistar rats by the explant method. Micromolar concentrations of dexamethasone, given as pretreatment for 48 hours, suppressed the ANF-mediated response. The dexamethasone-induced suppression was detectable at 6 hours and reached a maximum 24 hours after administration in a dose-dependent manner. Inhibitors of protein synthesis blocked this effect of the glucocorticoid. The basal activity of guanylate cyclase in the dexamethasone-treated cells was lower than in the control cells. Other steroids having glucocorticoid action mimicked this suppression of the ANF-mediated response. This suppression was blocked by a glucocorticoid receptor antagonist. The results suggest that glucocorticoids suppress ANF-mediated cGMP formation by VSMC through glucocorticoid type II receptors and the induction of protein synthesis. Suppression of the ANF-mediated response may play a role in glucocorticoid-induced hypertension.
K Yasunari, and
M Kohno, and
K Murakawa, and
K Yokokawa, and
T Takeda
March 1986,
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K Yasunari, and
M Kohno, and
K Murakawa, and
K Yokokawa, and
T Takeda
September 1985,
Life sciences,
K Yasunari, and
M Kohno, and
K Murakawa, and
K Yokokawa, and
T Takeda
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K Yasunari, and
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K Yasunari, and
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K Yasunari, and
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K Yasunari, and
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K Yasunari, and
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K Yasunari, and
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