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Immersion freezing of suspended particles and cells for cryo-electron microscopy. 2011

Guenter P Resch, and Marlene Brandstetter, and Lisa Königsmaier, and Edit Urban, and Angela M Pickl-Herk
IMP-IMBA-GMI Electron Microscopy Facility, Institute of Molecular Biotechnology, 1030 Vienna, Austria. guenter.resch@imba.oeaw.ac.at

UI MeSH Term Description Entries
D007101 Immersion The placing of a body or a part thereof into a liquid. Submersion,Immersions,Submersions
D002477 Cells The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary. Cell
D013535 Suspensions Colloids with liquid continuous phase and solid dispersed phase; the term is used loosely also for solid-in-gas (AEROSOLS) and other colloidal systems; water-insoluble drugs may be given as suspensions. Suspension
D015925 Cryopreservation Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens. Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic
D052638 Particulate Matter Particles of any solid substance, generally under 30 microns in size, often noted as PM30. There is special concern with PM1 which can get down to PULMONARY ALVEOLI and induce MACROPHAGE ACTIVATION and PHAGOCYTOSIS leading to FOREIGN BODY REACTION and LUNG DISEASES. Ultrafine Fiber,Ultrafine Fibers,Ultrafine Particle,Ultrafine Particles,Ultrafine Particulate Matter,Air Pollutants, Particulate,Airborne Particulate Matter,Ambient Particulate Matter,Fiber, Ultrafine,Particle, Ultrafine,Particles, Ultrafine,Particulate Air Pollutants,Particulate Matter, Airborne,Particulate Matter, Ambient,Particulate Matter, Ultrafine
D020285 Cryoelectron Microscopy Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains. Electron Cryomicroscopy,Cryo-electron Microscopy,Cryo electron Microscopy,Cryo-electron Microscopies,Cryoelectron Microscopies,Cryomicroscopies, Electron,Cryomicroscopy, Electron,Electron Cryomicroscopies,Microscopies, Cryo-electron,Microscopies, Cryoelectron,Microscopy, Cryo-electron,Microscopy, Cryoelectron

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