BIOMAT Database Logo BIOMAT Database Logo

Isolation, characterization and distribution of adenosine 3':5'-cyclic monophosphate from Pinus radiata. 1978

T Wilson, and E Moustafa, and A G Renwick

Cyclic AMP was extracted in 0.1 M-HCl from tissues of Pinus radiata and purified by gel filtration on Sephadex G-10, and chromatography on Dowex AG1 (X2) and polyethyleneimine-cellulose in two separate solvent systems. Presumptive cyclic AMP from 10kg batches of pine needles was characterized by countercurrent distribution in the presence of cyclic [8-3H]AMP. Statistical analysis of the curves for radioactivity and mass (determined by the Gilman competitive-binding assay) showed that the fit of the curves was highly significant for seven degrees of freedom. The distribution of cyclic AMP within P. radiata and various other plant tissues was determined by the Gilman procedure. The results suggest that there is no relationship between variations in cyclic AMP concentrations and the known function of the tissue in which it was measured.

UI MeSH Term Description Entries
D010944 Plants Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE. Plant
D002845 Chromatography Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts. Chromatographies
D003377 Countercurrent Distribution A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed) Chromatography, Countercurrent,Countercurrent Chromatography,Distribution, Countercurrent
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D014018 Tissue Distribution Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios. Distribution, Tissue,Distributions, Tissue,Tissue Distributions
D014197 Trees Woody, usually tall, perennial higher plants (Angiosperms, Gymnosperms, and some Pterophyta) having usually a main stem and numerous branches. Tree

Related Publications

T Wilson, and E Moustafa, and A G Renwick
Adenosine 3':5'-cyclic monophosphate in higher plants: Isolation and characterization of adenosine 3':5'-cyclic monophosphate from Kalanchoe and Agave.
July 1977, The Biochemical journal,
T Wilson, and E Moustafa, and A G Renwick
Adenosine 3':5'-Cyclic Monophosphate in Chlamydomonas reinhardtii: Isolation and Characterization.
April 1973, Proceedings of the National Academy of Sciences of the United States of America,
T Wilson, and E Moustafa, and A G Renwick
Synthesis and characterization of photoaffinity labels for adenosine 3':5'-cyclic monophosphate and adenosine 5'-monophosphate.
October 1973, Biochemistry,
T Wilson, and E Moustafa, and A G Renwick
Analysis of cyclic 3',5'-adenosine monophosphate and cyclic 3',5'-guanosine monophosphate.
January 1971, Methods of biochemical analysis,
T Wilson, and E Moustafa, and A G Renwick
[Cyclic adenosine 3',5'-monophosphate].
January 1972, Postepy biochemii,
T Wilson, and E Moustafa, and A G Renwick
Terferol, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase. I. Isolation and characterization.
January 1984, The Journal of antibiotics,
T Wilson, and E Moustafa, and A G Renwick
The endometrial adenosine cyclic 3':5'-monophosphate-dependent protein kinase. Distribution, subunit structure, and kinetics of adenosine cyclic 3':5'-monophosphate binding.
May 1973, The Journal of biological chemistry,
T Wilson, and E Moustafa, and A G Renwick
Simultaneous isolation of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) in small tissue samples.
May 1974, Analytical biochemistry,
T Wilson, and E Moustafa, and A G Renwick
Isolation of adenosine 3', 5'-monophosphate and guanosine 3', 5'-monophosphate from rat urine.
May 1963, Biochemical and biophysical research communications,
T Wilson, and E Moustafa, and A G Renwick
Control of Neurospora crassa morphology by cyclic adenosine 3', 5'-monophosphate and dibutyryl cyclic adenosine 3', 5'-monophosphate.
April 1976, Journal of bacteriology,
Copied contents to your clipboard!