Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells. 2011

Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE 68198-6805, USA.

Self-renewal is a complex biological process necessary for maintaining the pluripotency of embryonic stem cells (ESCs). Recent studies have used global proteomic techniques to identify proteins that associate with the master regulators Oct4, Nanog and Sox2 in ESCs or in ESCs during the early stages of differentiation. Through an unbiased proteomic screen, Banf1 was identified as a Sox2-associated protein. Banf1 has been shown to be essential for worm and fly development but, until now, its role in mammalian development and ESCs has not been explored. In this study, we examined the effect of knocking down Banf1 on ESCs. We demonstrate that the knockdown of Banf1 promotes the differentiation of mouse ESCs and decreases the survival of both mouse and human ESCs. For mouse ESCs, we demonstrate that knocking down Banf1 promotes their differentiation into cells that exhibit markers primarily associated with mesoderm and trophectoderm. Interestingly, knockdown of Banf1 disrupts the survival of human ESCs without significantly reducing the expression levels of the master regulators Sox2, Oct4 and Nanog or inducing the expression of markers of differentiation. Furthermore, we determined that the knockdown of Banf1 alters the cell cycle distribution of both human and mouse ESCs by causing an uncharacteristic increase in the proportion of cells in the G2-M phase of the cell cycle.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D050814 Octamer Transcription Factor-3 An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION. Oct-3 Transcription Factor,Transcription Factor Oct-3,Oct-4 Transcription Factor,Octamer-Binding Protein 4,POU Domain, Class 5, Transcription Factor 1,POU5F1 Transcription Factor,Transcription Factor Oct-4,Oct 3 Transcription Factor,Oct 4 Transcription Factor,Oct-3, Transcription Factor,Oct-4, Transcription Factor,Octamer Binding Protein 4,Octamer Transcription Factor 3,Transcription Factor Oct 3,Transcription Factor Oct 4,Transcription Factor, Oct-3,Transcription Factor, Oct-4,Transcription Factor, POU5F1,Transcription Factor-3, Octamer
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
March 2011, Journal of cellular biochemistry,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
August 2013, BMC cancer,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
May 2021, BMC cancer,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
January 2004, Stem cells (Dayton, Ohio),
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
September 2012, Experimental cell research,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
January 2012, Nature communications,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
January 2017, PloS one,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
February 2010, The Journal of biological chemistry,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
September 2015, The Journal of biological chemistry,
Jesse L Cox, and Sunil K Mallanna, and Briana D Ormsbee, and Michelle Desler, and Matthew S Wiebe, and Angie Rizzino
August 2008, Journal of cell science,
Copied contents to your clipboard!