Automatic computation of enzyme kinetics by HPLC. 1990

P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
Dipartimento di Scienze e Tecnologie Biomediche, University of Milano, Italy.

Enzyme activity can be easily measured by HPLC using traces of the product itself as an internal standard. Our procedure involved the development of an equation using the experimental data obtained in the kinetic assay. The entire procedure can thus be automated and a computer program is presented here for facilitating the assay and saving time. The determination of the activity of NAD kinase is reported as an example.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008432 Mathematical Computing Computer-assisted interpretation and analysis of various mathematical functions related to a particular problem. Statistical Computing,Computing, Statistical,Mathematic Computing,Statistical Programs, Computer Based,Computing, Mathematic,Computing, Mathematical,Computings, Mathematic,Computings, Mathematical,Computings, Statistical,Mathematic Computings,Mathematical Computings,Statistical Computings
D010770 Phosphotransferases A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7. Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D011381 Programming Languages Specific languages used to prepare computer programs. Language, Programming,Languages, Programming,Programming Language
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004798 Enzymes Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified. Biocatalyst,Enzyme,Biocatalysts
D000465 Algorithms A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task. Algorithm
D012985 Software Design Specifications and instructions applied to the software. Flowcharts (Computer),Flow Charts (Computer),Flowcharts,Chart, Flow (Computer),Charts, Flow (Computer),Design, Software,Designs, Software,Flow Chart (Computer),Flowchart,Flowchart (Computer),Software Designs
D017853 Phosphotransferases (Alcohol Group Acceptor) A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
May 1976, The Biochemical journal,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
June 1963, Klinische Wochenschrift,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
November 1969, The Biochemical journal,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
July 1974, ALAFO; revista de la Asociacion Latinoamericana de Facultades de Odontologia,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
August 1981, Applied optics,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
January 1949, Nature,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
July 1969, Archives des maladies du coeur et des vaisseaux,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
April 1972, Nihon Igaku Hoshasen Gakkai zasshi. Nippon acta radiologica,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
November 1980, Applied optics,
P L Mauri, and M Bitonti, and M Pace, and P G Pietta, and D Agnellini
February 1964, Analytical biochemistry,
Copied contents to your clipboard!