Biomaterial Database

Mathematical description of electrophoretic separation of weak acids by means of an H+ pulse. 1990

P Gebauer, and M Deml, and J Pospichal, and P Bocek

Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Brno.

The paper gives a detailed theoretical description of the recently published principle of separation by a dynamic pulse in zone electrophoresis. The theory is illustrated by using a system in which weak acids are separated by migrating against an H+ pulse. The mathematical description pertains to (i) the evolution and migration of the concentration profile of the H+ pulse, and (ii) the migration of a sample substance (here a weak acid) against and through the pulse. As a result the migration (detection) times of the separated sample components are obtained. Based on the theory, a graphical treatment was developed which allows fast optimization of the separation conditions by observing (on the PC monitor) how the trajectories of the substances (drawn in a time vs. distance coordinate system) change with a change in the parameters of the pulse. Some of the results illustrate the possible advantages of using the dynamic pulse method in analytical practice.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D008962	Models, Theoretical	Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.	Experimental Model,Experimental Models,Mathematical Model,Model, Experimental,Models (Theoretical),Models, Experimental,Models, Theoretic,Theoretical Study,Mathematical Models,Model (Theoretical),Model, Mathematical,Model, Theoretical,Models, Mathematical,Studies, Theoretical,Study, Theoretical,Theoretical Model,Theoretical Models,Theoretical Studies
D011522	Protons	Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.	Hydrogen Ions,Hydrogen Ion,Ion, Hydrogen,Ions, Hydrogen,Proton
D004586	Electrophoresis	An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.	Electrophoreses
D000143	Acids	Chemical compounds which yield hydrogen ions or protons when dissolved in water, whose hydrogen can be replaced by metals or basic radicals, or which react with bases to form salts and water (neutralization). An extension of the term includes substances dissolved in media other than water. (Grant & Hackh's Chemical Dictionary, 5th ed)	Acid