BIOMAT Database Logo BIOMAT Database Logo

Detection and typing of human papillomavirus using the Vira Type "in situ" kit: comparison with a conventional dot blot technique. 1990

B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Microbiology, Department Royal Women's Hospital, Carlton, Victoria, Australia.

A new commercial kit (Vira Type "in situ", Life Technologies, Inc., Molecular Diagnostics Division, Guithersburg, Maryland, USA) for the detection of human papillomavirus (HPV) types 6, 11, 16, 18, 31, 33 and 35 in routinely processed human anogenital tissue was compared with a conventional dot blot assay for HPV 6, 11, 16 and 18. Both systems use double-stranded genomic DNA probes for the detection of type specific HPV DNA. The probes used on the dot blots were labelled with 32P and visualised autoradiographically. The Vira Type probes were labelled with biotin and visualised using a streptavidin-alkaline phosphatase conjugate with NBT-BCIP substrate. Biopsy specimens from the cervix, vagina, and vulva of 46 women were processed by both methods and compared. The histological diagnoses ranged from benign changes, to dysplasia, and invasive carcinoma. Overall, 50% of biopsy specimens were positive for HPV DNA by dot blot hybridisation; only 39% were positive by Vira Type in situ hybridisation. Three of the specimens positive by the Vira Type "in situ" kit showed no cross hybridisation and were the same HPV type as the dot blot. A further 13 showed hybridisation, but the showed cross hybridisation, but the to the dot blot results. One biopsy specimen was positive for different HPV types by the two tests and one was positive by Vira Type and negative by dot blot. Six biopsy specimens were negative by Vira Type but positive by dot blot. It is concluded that the Vira Type "in situ" kit has a similar specificity but lower sensitivity than the dot blot hybridisation method for the detection of HPV DNA.

UI MeSH Term Description Entries
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D011933 Reagent Kits, Diagnostic Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use. Diagnostic Reagent Kits,Diagnostic Reagents and Test Kits,Diagnostic Test Kits,In Vitro Diagnostic Device,In Vitro Diagnostic Devices,In Vitro Diagnostic Medical Device,In Vitro Diagnostic Medical Devices,Kits, Diagnostic Reagent,Diagnostic Reagent Kit,Diagnostic Test Kit,Kit, Diagnostic Reagent,Kit, Diagnostic Test,Kits, Diagnostic Test,Reagent Kit, Diagnostic,Test Kit, Diagnostic,Test Kits, Diagnostic
D002578 Uterine Cervical Dysplasia Abnormal development of immature squamous EPITHELIAL CELLS of the UTERINE CERVIX, a term used to describe premalignant cytological changes in the cervical EPITHELIUM. These atypical cells do not penetrate the epithelial BASEMENT MEMBRANE. Cervical Dysplasia,Cervical Intraepithelial Neoplasia,Cervix Dysplasia,Dysplasia of Cervix Uteri,Neoplasia, Cervical Intraepithelial,Cervical Intraepithelial Neoplasia, Grade III,Cervical Intraepithelial Neoplasms,Intraepithelial Neoplasia, Cervical,Cervical Dysplasia, Uterine,Cervical Dysplasias,Cervical Intraepithelial Neoplasm,Cervix Uteri Dysplasia,Cervix Uteri Dysplasias,Dysplasia, Cervical,Dysplasia, Cervix,Dysplasia, Uterine Cervical,Intraepithelial Neoplasm, Cervical,Intraepithelial Neoplasms, Cervical,Neoplasm, Cervical Intraepithelial,Neoplasms, Cervical Intraepithelial
D002583 Uterine Cervical Neoplasms Tumors or cancer of the UTERINE CERVIX. Cancer of Cervix,Cancer of the Cervix,Cancer of the Uterine Cervix,Cervical Cancer,Cervical Neoplasms,Cervix Cancer,Cervix Neoplasms,Neoplasms, Cervical,Neoplasms, Cervix,Uterine Cervical Cancer,Cancer, Cervical,Cancer, Cervix,Cancer, Uterine Cervical,Cervical Cancer, Uterine,Cervical Cancers,Cervical Neoplasm,Cervical Neoplasm, Uterine,Cervix Neoplasm,Neoplasm, Cervix,Neoplasm, Uterine Cervical,Uterine Cervical Cancers,Uterine Cervical Neoplasm
D002584 Cervix Uteri The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal. Cervical Canal of the Uterus,Cervical Canal, Uterine,Ectocervix,Endocervical Canal,Endocervix,External Os Cervix,External Os of the Cervix,Uterine Cervical Canal,Cervix,Cervixes,Uterine Cervix,Canal, Endocervical,Canal, Uterine Cervical,Cervix, External Os,Cervix, Uterine,Endocervical Canals,Uterine Cervical Canals
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D014621 Vagina The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed) Vaginas

Related Publications

B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Human papillomavirus detection by PCR and typing by dot-blot.
January 1999, Methods in molecular medicine,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Detection of human papillomavirus DNA in the normal cervices of Japanese women by the dot-blot (Vira Pap) method.
September 1992, Asia-Oceania journal of obstetrics and gynaecology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
[Detection of papilloma virus infection in the human uterine cervix by dot blot (Vira Pap) method in comparison with Southern blot method].
January 1989, Rinsho byori. The Japanese journal of clinical pathology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Human papillomavirus DNA in anal carcinomas. Comparison of in situ and dot blot hybridization.
September 1991, American journal of clinical pathology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Detection and typing of human papillomavirus infection of the uterine cervix by dot blot hybridisation: comparison of scrapes and biopsies.
April 1989, Journal of medical virology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Detection of human papillomavirus (HPV) using dot blot and Southern blot, hybridizing with a mixture of seven probes.
April 1992, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
[Detection of papilloma virus infection in the human uterine cervix by dot blot (Vira Pap) method. Comparison with smear diagnosis].
September 1988, Rinsho byori. The Japanese journal of clinical pathology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Detection and typing of human papillomavirus DNA in the uterine cervix of Japanese women by nonradioactive dot blot and Southern blot hybridization.
January 1993, Diagnostic cytopathology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
A comparison of human papillomavirus detection rates by dot blot assay from smear and biopsy specimens with regard to human papillomavirus type and histologic diagnosis.
June 1994, American journal of clinical pathology,
B E Faulkner-Jones, and V M Bellomarino, and A J Borg, and K Orzeszko, and S M Garland
Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation.
October 1992, Journal of clinical pathology,
Copied contents to your clipboard!