Biomaterial Database

Use of the PBS2 uracil-DNA glycosylase inhibitor to differentiate the uracil-DNA glycosylase activities encoded by herpes simplex virus types 1 and 2. 1990

T A Winters, and M V Williams

Department of Medical Microbiology and Immunology, Ohio State University, Columbus 43210.

The bacteriophage PBS2 encoded uracil-DNA glycosylase (UNG) inhibitor was examined for its effect upon the nuclear UNG activities of KB, HeLa, and Vero cells infected with herpes simplex virus (HSV) type 1 or 2 and mock-infected cells. UNG activity from HSV-1 infected cells exhibited the greatest sensitivity to inhibition by the inhibitor, while UNG activity from cells infected with HSV-2 exhibited the greatest resistance. This differential effect was dependent upon the virus, cell line, and buffer system used in the reaction. Furthermore, the PBS2 UNG inhibitor's differential effect, provides a means of distinguishing the herpesvirus UNG activities from one another, and from the cellular UNG activity. Therefore, this method of identification should prove to be useful for the purification and characterization of the viral enzymes from infected cell nuclear extracts.

UI	MeSH Term	Description	Entries
D009699	N-Glycosyl Hydrolases	A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.	Glycoside Hydrolases, Nitrogen-linked,Hydrolases, N-Glycosyl,Nucleosidase,Nucleosidases,Nucleoside Hydrolase,Nitrogen-linked Glycoside Hydrolases,Nucleoside Hydrolases,Glycoside Hydrolases, Nitrogen linked,Hydrolase, Nucleoside,Hydrolases, N Glycosyl,Hydrolases, Nitrogen-linked Glycoside,Hydrolases, Nucleoside,N Glycosyl Hydrolases,Nitrogen linked Glycoside Hydrolases
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006561	Herpes Simplex	A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)	Herpes Simplex Virus Infection
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D045647	DNA Glycosylases	A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.	DNA N-glycosidase,DNA Glycosylase,Methylpurine DNA Glycosylase,DNA Glycosylase, Methylpurine,DNA N glycosidase,Glycosylase, DNA,Glycosylase, Methylpurine DNA,Glycosylases, DNA
D051981	Uracil-DNA Glycosidase	An enzyme that catalyzes the HYDROLYSIS of the N-glycosidic bond between sugar phosphate backbone and URACIL residue during DNA synthesis.	Ung DNA Glycosylase,Ura-DNA Glycosidase,Ura-DNA Glycosylase,Uracil DNA Glycosylase,Uracil N-Glycosidase,Uracil N-Glycosylase,Uracil-DNA Glycosylase,DNA Glycosylase, Ung,DNA Glycosylase, Uracil,Glycosidase, Ura-DNA,Glycosidase, Uracil-DNA,Glycosylase, Ung DNA,Glycosylase, Ura-DNA,Glycosylase, Uracil DNA,Glycosylase, Uracil-DNA,N-Glycosidase, Uracil,N-Glycosylase, Uracil,Ura DNA Glycosidase,Ura DNA Glycosylase,Uracil DNA Glycosidase,Uracil N Glycosidase,Uracil N Glycosylase
D018139	Simplexvirus	A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.	Herpes Simplex Virus,Herpesvirus 1, Saimiriine,Herpesvirus 1, Saimirine,Herpesvirus 16, Cercopithecine,Marmoset Virus,Cercopithecine Herpesvirus 16,Herpes Labialis Virus,Herpes-T Virus,Herpesvirus 1 (alpha), Saimirine,Herpesvirus Hominis,Herpesvirus Papio 2,Herpesvirus Platyrhinae,Marmoset Herpesvirus,Saimiriine Herpesvirus 1,Herpes Labialis Viruses,Herpes Simplex Viruses,Herpes T Virus,Herpes-T Viruses,Herpesvirus Homini,Herpesvirus, Marmoset,Herpesviruses, Marmoset,Homini, Herpesvirus,Hominis, Herpesvirus,Labialis Virus, Herpes,Labialis Viruses, Herpes,Marmoset Herpesviruses,Marmoset Viruses,Platyrhinae, Herpesvirus,Saimirine Herpesvirus 1,Simplexviruses,Virus, Herpes Labialis,Viruses, Herpes Labialis