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Chromosomal localization of the human myeloperoxidase gene by in situ hybridization using oligonucleotide probes. 1990
S R Zaki, and
G E Austin, and
W C Chan, and
A L Conaty, and
S Trusler, and
S Trappier, and
R B Lindsey, and
D C Swan
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
Oligonucleotide probes have been used to map the myeloperoxidase (MPO) gene locus to chromosome bands 17q21-22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72-base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium-labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.
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