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Development of methods for the manipulation of the genomes of parasitic protozoa will lead to enhanced understanding of parasite biology and host-parasite relationships. Efficient gene transfer and targeted integration by homologous recombination were achieved in the parasitic protozoan Trypanosoma brucei, the causative agent of sleeping sickness. An expression vector with the neomycin phosphotransferase gene (neo), under the control of a procyclic acidic repetitive protein (PARP) gene promoter, was targeted into an intergenic region in beta alpha-tubulin-gene tandem array. Sixteen copies of neo were found in a tandem array in one of the transfectants where the PARP promoter controlled alpha-amanitin-resistant transcription of neo, whereas transcription of tubulin genes remained alpha-amanitin-sensitive.
M G Lee, and
L H Van der Ploeg
March 1991,
Proceedings of the National Academy of Sciences of the United States of America,
M G Lee, and
L H Van der Ploeg
September 2002,
Molecular microbiology,
M G Lee, and
L H Van der Ploeg
May 2013,
BMC microbiology,
M G Lee, and
L H Van der Ploeg
December 2016,
Acta tropica,
M G Lee, and
L H Van der Ploeg
May 2007,
Molecular and biochemical parasitology,
M G Lee, and
L H Van der Ploeg
November 1999,
Genes & development,
M G Lee, and
L H Van der Ploeg
February 1996,
Nucleic acids research,
M G Lee, and
L H Van der Ploeg
September 1997,
Biological chemistry,
M G Lee, and
L H Van der Ploeg
January 2020,
Methods in molecular biology (Clifton, N.J.),
M G Lee, and
L H Van der Ploeg
April 1993,
The Biochemical journal,
