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Tubby-tagged balancers for the Drosophila X and second chromosomes. 2011
Dipartimento di Biologia e Biotecnologie “Charles Darwin” and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza, Università di Roma, Rome, Italy.
We generated FM7a and CyO balancer chromosomes bearing a Tubby1 (Tb1) dominant transgene. Flies heterozygous for these FM7a and CyO derivatives exhibit a phenotype undistinguishable from that elicited by the Tb1 mutation associated with the TM6B balancer. We tested two of these Tb-bearing balancers (FM7-TbA and CyO-TbA) for more than 30 generations and found that the Tb1 transgene they carry is stable. Thus, these new Tb-tagged balancers are particularly useful for balancing lethal mutations and distinguish homozygous mutant larvae from their heterozygous siblings.
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