-transparent.png){kind=logo}
To study the growth and morphology of feline mammary epithelial cells in vitro, normal feline mammary tissues as well as cells collected from feline milk were cultured and examined using phase-contrast microscopy and immunohistochemistry. The method employed was a modification of Hiratsuka et al., and was found to be useful for the culture of dissociated feline mammary tissue. Small polygonal cells and large spindle-shaped cells that were positively stained with anti-keratin and anti-actin antibodies formed colonies with a pavement-like structure in culture of mid-pregnant mammary gland. Cultures of involuting mammary gland were composed mainly of small spindle-shaped cells, which were negative for keratin immunostaining and thought to be fibroblasts. Myofibroblasts, which participate in feline mammary carcinogenesis, and show keratin negative, actin positive, were not encountered in the present culture system of normal mammary gland. The cells from milk formed small colonies, but did not grow to reach confluent monolayer.
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
February 1977,
The Veterinary clinics of North America,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
October 2008,
Journal of feline medicine and surgery,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
May 1996,
Nature medicine,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
September 2003,
The Veterinary record,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
February 2009,
Veterinary journal (London, England : 1997),
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
September 2004,
Veterinary journal (London, England : 1997),
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
January 1973,
In vitro,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
November 1956,
Revue belge de pathologie et de medecine experimentale,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
January 1964,
Acta - Unio Internationalis Contra Cancrum,
S Tateyama, and
A Takeshita, and
D Nosaka, and
R Yamaguchi, and
N Miyoshi, and
F Kondo
January 1981,
Methods in enzymology,
