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Poliovirus-specific double-stranded RNA molecules containing covalently attached protein were coupled with a biotin ester through the protein moiety. Subsequent interaction of the RNA-biotin with avidin attached to electronopaque plastic spheres led to the formation of complexes that were easily visualized in the electron microscope. Avidinspheres were associated only with one end of the RNA-biotin molecules, as seen in the electron microscope. Avidin-sphere attachment to poliovirus double-stranded RNA is strand specific, as shown by molecular hybridization of strand-specific probes to the separated strands of denatured complexes. [3H]DNA complementary to polio virion RNA hybridized exclusively to the strands bearing associated spheres [(+) strands] whereas 125I-labeled virion RNA hybridized predominantly with strands without spheres [(-)strands]. This biotin-avidin labeling technique provides a means for the isolation of full-length poliovirus (-) strands and may provide a general means for isolation of double-stranded polynucleotides containing tightly attached protein.
O C Richards, and
E Ehrenfeld, and
J Manning
July 1981,
Journal of virology,
O C Richards, and
E Ehrenfeld, and
J Manning
March 1964,
Science (New York, N.Y.),
O C Richards, and
E Ehrenfeld, and
J Manning
November 1964,
Virology,
O C Richards, and
E Ehrenfeld, and
J Manning
January 1974,
Biochemical and biophysical research communications,
O C Richards, and
E Ehrenfeld, and
J Manning
September 1978,
Journal of virology,
O C Richards, and
E Ehrenfeld, and
J Manning
February 2021,
Methods and protocols,
O C Richards, and
E Ehrenfeld, and
J Manning
January 1975,
Archives of virology,
O C Richards, and
E Ehrenfeld, and
J Manning
January 1972,
Archives of biochemistry and biophysics,
O C Richards, and
E Ehrenfeld, and
J Manning
April 1970,
Virology,
O C Richards, and
E Ehrenfeld, and
J Manning
October 1965,
Journal of biochemistry,
