BIOMAT Database Logo BIOMAT Database Logo

Combined time-lapse cinematography and immuno-electron microscopy. 1990

B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Clinical Research Centre, Harrow, Middlesex, England.

A method was developed to record interactions between mobile non-adherent immunocytes by time-lapse cinematography and then to study the same cells by immuno-electron microscopy, using monoclonal antibodies against surface components. For this purpose a modified stage was designed to fit an inverted microscope. The attachment included a device to cool the culture chamber with N2 gas, a micro-injector for monoclonal antibody and immuno-gold treatment, and two pairs of washing needles to change the medium without disturbance. The technique was first employed to study the formation of aggregates around the antigen-presenting cells in cultures containing cells from hyper-immunized animals. Recently peripheral blood cells from normal subjects and patients with immune deficiency syndromes were stimulated with pokeweed mitogen, cluster formation was recorded, and the cells were processed for immuno-electron microscopy.

UI MeSH Term Description Entries
D007158 Immunologic Techniques Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies. Antibody Dissociation,Immunologic Technic,Immunologic Technics,Immunologic Technique,Immunological Technics,Immunological Techniques,Technic, Immunologic,Technics, Immunologic,Technique, Immunologic,Techniques, Immunologic,Antibody Dissociations,Dissociation, Antibody,Dissociations, Antibody,Immunological Technic,Immunological Technique,Technic, Immunological,Technics, Immunological,Technique, Immunological,Techniques, Immunological
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009040 Motion Pictures The art, technique, or business of producing motion pictures for entertainment, propaganda, or instruction. Film,Motion Picture,Movie,Cinema,Films,Films as Topic,Movies,Movies as Topic,Cinemas,Films as Topics,Movies as Topics,Picture, Motion,Pictures, Motion
D002450 Cell Communication Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP. Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000938 Antigen-Presenting Cells A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors. Accessory Cells, Immunologic,Antigen-Presenting Cell,Immunologic Accessory Cells,Accessory Cell, Immunologic,Cell, Immunologic Accessory,Cells, Immunologic Accessory,Immunologic Accessory Cell,Antigen Presenting Cell,Antigen Presenting Cells,Cell, Antigen-Presenting,Cells, Antigen-Presenting
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinematography of vancomycin--treated microbial cells.
April 1967, Canadian journal of microbiology,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinematography study of preimplantation mouse embryo development.
January 2009, Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinematography studies of cell cycle and mitosis duration.
May 1977, Experimental cell research,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Studies of gliomas in tissue culture, using time-lapse cinematography.
July 1960, Journal of neuropathology and experimental neurology,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinematography in living Drosophila tissues: preparation of material.
November 2006, CSH protocols,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinemicrography and scanning electron microscopy of platelet formation by megakaryocytes.
January 1983, Blood cells,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Time-lapse cinematography of the capillary tube cell migration inhibition test.
January 1980, International archives of allergy and applied immunology,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Embryo through the lens: from time-lapse cinematography to artificial intelligence.
February 2020, Fertility and sterility,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Morphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes.
May 2021, Theriogenology,
B M Balfour, and T Goscicka, and J L MacKenzie, and A Gautam, and M Tate, and J Clark
Neuroblastoma cell differentiation: a tissue culture study using time-lapse cinematography.
January 1973, Neurobiology,
Copied contents to your clipboard!