Biomaterial Database

A multiple biomarker approach to investigate the effects of copper on the marine bivalve mollusc, Mytilus edulis. 2011

Sherain N Al-Subiai, and A John Moody, and Sanaa A Mustafa, and Awadhesh N Jha

Ecotoxicology Research and Innovation Centre, School of Biomedical and Biological Sciences, University of Plymouth, Plymouth, PL4 8AA, UK.

While copper (Cu) is considered to be an essential trace element for many organisms, overexposure to this metal can induce a wide spectrum of effects including DNA damage. Given that Cu is a highly relevant contaminant in the marine environment, we aimed to evaluate the induction of DNA strand breaks (using the comet assay) in haemocytes and concurrently also determined biological responses at higher levels of biological organisation in bivalve molluscs, Mytilus edulis, following exposure for 5 days to a range of environmentally realistic levels of Cu (18-56 μg l(-1)). Prior to evaluation of genetic damage, the maximum tolerated concentration (MTC) was also determined, which was found to be (100 μg l(-1)) above which complete mortality over the exposure period was observed. In addition to DNA damage, levels of glutathione in adductor muscle extracts, histopathological examination of various organs (viz., adductor muscle, gills and digestive glands) and clearance rates as a physiological measure at individual level were also determined. Furthermore, tissue-specific accumulation and levels of Cu in water samples were also determined using ICP-MS. There was a strong concentration-dependant induction for DNA damage and total glutathione levels increased by 1.8-fold at 56 μg l(-1) Cu. Histological examination of the organs showed qualitatively distinct abnormalities. Clearance rate also showed a significant decrease compared to controls even at the lowest concentration (i.e. 18 μg l(-1); P=0.003). Cu levels in adductor muscle (P=0.012), digestive gland (P=0.008) and gills (P=0.002) were significantly higher than in the control. The multi-biomarker approach used here suggests that in some cases clear relationships exist between genotoxic and higher level effects, which could be adopted as an integrated tool to evaluate different short and long-term toxic effects of pollutants.

UI	MeSH Term	Description	Entries
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D004249	DNA Damage	Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.	DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004781	Environmental Exposure	The exposure to potentially harmful chemical, physical, or biological agents in the environment or to environmental factors that may include ionizing radiation, pathogenic organisms, or toxic chemicals.	Exposure, Environmental,Environmental Exposures,Exposures, Environmental
D005880	Gills	Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.	Gill
D005978	Glutathione	A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.	Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D006434	Hemocytes	Any blood or formed element especially in invertebrates.	Hemocyte
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012623	Seawater	The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.	Sea Water,Sea Waters,Seawaters,Water, Sea,Waters, Sea
D014018	Tissue Distribution	Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.	Distribution, Tissue,Distributions, Tissue,Tissue Distributions