Sensitive competitive flow injection chemiluminescence immunoassay for IgG using gold nanoparticle as label. 2011

Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, People's Republic of China. honglanqi@snnu.edu.cn

A sensitive competitive flow injection chemiluminescence (CL-FIA) immunoassay for immunoglobulin G (IgG) was developed using gold nanoparticle as CL label. In the configuration, anti-IgG antibody was immobilized on a glass capillary column surface by 3-(aminopropyl)-triethoxysilane and glutaraldehyde to form immunoaffinity column. Analyte IgG and gold nanoparticle labeled IgG were passed through the immunoaffinity column mounted in a flow system and competed for the surface-confined anti-IgG antibody. CL emission was generated from the reaction between luminol and hydrogen peroxide in the presence of Au (III), generated from chemically oxidative dissolution of gold nanoparticle by an injection of 0.10 mol L(-1) HCl-0.10 mol L(-1) NaCl solution containing 0.10 mmol L(-1) Br(2). The concentration of analyte IgG was inversely related to the amount of bound gold nanoparticle labeled IgG and the CL intensity was linear with the concentration of analyte IgG from 1.0 ng mL(-1) to 40 ng mL(-1) with a detection limit of 5.2×10(-10) gm L(-1). The whole assay time including the injections and washing steps was only 30 min for one sample, which was competitive with CL immunoassays based on a gold nanoparticle label and magnetic separation. This work demonstrates that the CL immunoassay incorporation of nanoparticle label and flow injection is promising for clinical assay with sensitivity and high-speed.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D001966 Bromine A halogen with the atomic symbol Br, atomic number 35, and atomic weight 79.904. It is a volatile reddish-brown liquid that gives off suffocating vapors, is corrosive to the skin, and may cause severe gastroenteritis if ingested. Bromine-79,Bromine 79
D002021 Buffers A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer. Buffer
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D005898 Glass Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies

Related Publications

Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
October 2014, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
September 2020, Luminescence : the journal of biological and chemical luminescence,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
November 2023, Analytical chemistry,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
December 2017, Mikrochimica acta,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
February 2011, Chemistry (Weinheim an der Bergstrasse, Germany),
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
September 2008, The Analyst,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
May 2004, Applied biochemistry and biotechnology,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
February 2018, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
March 2006, Analytical and bioanalytical chemistry,
Honglan Qi, and Li Shangguan, and Lin Liang, and Chen Ling, and Qiang Gao, and Chengxiao Zhang
January 1997, Biomedical chromatography : BMC,
Copied contents to your clipboard!