[The effects of prolonged sevoflurane anesthesia on renal function in liver fibrosis rabbits]. 2011

Wei-Yi Zhang, and Ai-Min Yang, and Yan Yin, and Deng-Feng Fang, and Yu Li
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.

OBJECTIVE To determine the effects of prolonged sevoflurane anesthesia on renal function in liver fibrosis rabbits. METHODS Fifty male new zealand rabbits were randomly divided into two groups: fibrosis group (n = 40) and normal control group (Group N, n = 10). In the fibrosis group, experimental liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4 soluted in oil with the concentration of 500 mL/L). Twelve weeks later, 24 rabbits survived in fibrosis group were randomly allocated to control group (Group F, n = 12) or treating with sevoflurane (Group S, n = 12). Both group S and group N received 1.0 minimum alveolar concentration (MAC) sevoflurane for 8 h with a fresh gas flow rate of 1 L/min. Group F was just given fluid infusion. After 8 h of 1.0 MAC anesthesia, sevoflurane was discontinued. Blood samples were collected before (T0) and 8 h (T8), 24 h (T24), 72 h (T72), and 120 h (T120) after anesthesia. The renal function was assessed by blood urea nitrogen (BUN) and creatinine (Cr). The serum inorganic fluoride was measured by ion-selective electrode. The pathological consequences of the livers were obtained after 5 days or when the rabbits died. RESULTS All the rabbits in group S and group F developed liver fibrosis. The hepatic function was significantly abnormal in group S and group F. Aminotransferase (AST), glutamic-pyruvic transaminase (ALT), lactate dehydrogenase (LDH) and total bile acid (TBA) were significantly higher in the fibrosis group (group S and group F) than those in group N. Alkaline phosphatase (ALP) in the fibrosis group was lower than that in group N. There were no significant differences of hepatic function between group S and group F. There were no significant differences in renal function and serum inorganic fluoride before and after anesthesia among the three groups. In group N and S, BUN and serum inorganic fluoride increased slightly at T8, and serum Cr was highest at T72. All of them returned to basic line at T120. In group F, BUN increased slightly at T8, and serum Cr was highest at T72. Serum inorganic fluoride was highest at T120. BUN and serum Cr returned to basic line at T120. CONCLUSIONS Prolonged sevoflurane anesthesia has no significant influences on renal function in normal and liver fibrosis rabbits.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D007677 Kidney Function Tests Laboratory tests used to evaluate how well the kidneys are working through examination of blood and urine. Function Test, Kidney,Function Tests, Kidney,Kidney Function Test,Test, Kidney Function,Tests, Kidney Function
D008103 Liver Cirrhosis Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules. Cirrhosis, Liver,Fibrosis, Liver,Hepatic Cirrhosis,Liver Fibrosis,Cirrhosis, Hepatic
D008297 Male Males
D008738 Methyl Ethers A group of compounds that contain the general formula R-OCH3. Ethers, Methyl
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D002251 Carbon Tetrachloride A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed) Tetrachloromethane,Tetrachloride, Carbon
D000077149 Sevoflurane A non-explosive inhalation anesthetic used in the induction and maintenance of general anesthesia. It does not cause respiratory irritation and may also prevent PLATELET AGGREGATION. BAX 3084,Fluoromethyl Hexafluoroisopropyl Ether,Fluoromethyl-2,2,2-trifluoro-1-(trifluoromethyl)ethyl Ether,Sevorane,Ultane
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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