BIOMAT Database Logo BIOMAT Database Logo

[Establishment and primary application of sandwich ELISA method to detect tenascin-C]. 2011

Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
Department of Orthopaedics, Tangdu Hospital, Fourth Miliatry Medical University, Xi'an 710038, China.

OBJECTIVE To establish a sandwich method to detect tenascin-c on the basis of preparation of monoclonal antibodies (mAbs) against tenascin-C (TN-C). METHODS The ascites of three stains of mAbs (No. 1A8, 3H7 and 4D6) were prepared and purified. The mAbs were conjugated with HRP and paired, respectively. The recombinant TN-C was taken as standard to analyze the optimal combination between mAbs. The sera TN-C concentrations of patients with osteosarocoma and the normal persons were evaluated with the sandwich ELISA method. RESULTS Among these mAbs, the sensitivity was obtained when combined the coated 1A8 with HRP-4D6. The sera TN-C significantly higher than the normal controls. CONCLUSIONS The sandwich ELISA method to detect TN-C was established successfully. The sera TN-C concentrations of patients with osteosarcoma and the normal persons were found distinct with the sandwich method.

UI MeSH Term Description Entries
D008297 Male Males
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D012516 Osteosarcoma A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed) Sarcoma, Osteogenic,Osteogenic Sarcoma,Osteosarcoma Tumor,Osteogenic Sarcomas,Osteosarcoma Tumors,Osteosarcomas,Sarcomas, Osteogenic,Tumor, Osteosarcoma,Tumors, Osteosarcoma

Related Publications

Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
[Establishment and evaluation of a double antibody sandwich ELISA to detect Csa2 protein of Candida albicans].
July 2014, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application].
March 2003, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
How to detect and purify tenascin-C.
January 2018, Methods in cell biology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
Application of Bead-ELISA method to detect Helicobacter pylori VacA.
February 1999, Microbial pathogenesis,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
[Establishment and application of double-antibody sandwich ELISA for determination of 3-nitrotyrosine].
November 2009, Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
Establishment of swine interleukin-6 sandwich ELISA.
March 2005, Comparative immunology, microbiology and infectious diseases,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
[Establishment of a sandwich ELISA method for detection of reporter chloramphenicol acetyltransferase gene].
March 2002, Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
[Preparation of monoclonal antibody against Pfs25 protein and establishment of double antibody sandwich ELISA method].
December 2011, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
Purification of HRSV F protein from a eukaryotic expression vector and establishment of a sandwich ELISA method.
July 2012, Molecular medicine reports,
Yu-cai Wang, and Dian-Zhong Zhang, and Li-hong He, and Yong Ding, and Le-qun Shan
Development of sandwich ELISA and lateral flow immunoassay to detect almond in processed food.
March 2022, Food chemistry,
Copied contents to your clipboard!