BIOMAT Database Logo BIOMAT Database Logo

In vitro study of transdermal penetration and iontophoresis of hepatitis B vaccines through rat skin. 2011

Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China.

In vitro percutaneous delivery of hepatitis B vaccines was investigated in order to assess the penetration of vaccine under passive diffusion and iontophoresis conditions. The study was carried out using Franz vertical diffusion cell through the hairless abdominal skin of Sprague-Dawley (SD) rats. Enzyme-linked immunosorbent assay (ELISA) was used to determine the cumulative amount of permeation and the retention amount of drug in skin. Passive diffusion alone resulted in less skin permeation and retention of hepatitis B vaccines, only (2.1 +/- 0.1) ng x cm(-2) and (2.3 +/- 0.1) ng x cm(-2) after 24 h when the initial concentration of vaccine in the donor compartment was 23 microg x mL(-1) and 46 microg x mL(-1), respectively. After removing the stratum corneum, the permeation and retention amount of hepatitis B vaccines increased to (383.7 +/- 86.2) ng x cm(-2) and (16.8 +/- 4.6) ng x cm(-2), respectively, 171.6-folds and 2.1-folds more than that from its intact skin with the drug loaded at 46 microg x mL(-1). Iontophoresis induced a significant increase of cumulative and retention amount of hepatitis B vaccines through the skin (P < 0.05). Application of iontophoresis significantly enhanced the permeation of hepatitis B vaccines (P < 0.05) by 2.7-folds and 6.6-folds for the intact skin, and by 1.6-folds and 1.8-folds for the tape-stripped skin with initial drug loading of 23 microg x mL(-1) and 46 microg x mL(-1), respectively. Iontophoresis also significantly increased the amount of drug retained in the skin. After applying iontophoresis for 6 h, the amount of skin retention was nearly the same as passive diffusion for 24 h both from intact skin [(16.8 +/- 4.6) ng x cm(-2) vs (13.3 +/- 5.4) ng x cm(-2)] (P > 0.05) and tape-stripped skin [(36.7 +/- 14.1) ng x cm(-2) vs (26.8 +/- 11.2) ng x cm(-2)] (P > 0.05). Overall, these findings revealed that the transportation efficiency of bioactive substance like hepatitis B vaccines may be improved by iontophoresis, which can be potentially used in the field of transcutaneous immunization.

UI MeSH Term Description Entries
D007478 Iontophoresis Therapeutic introduction of ions of soluble salts into tissues by means of electric current. In medical literature it is commonly used to indicate the process of increasing the penetration of drugs into surface tissues by the application of electric current. It has nothing to do with ION EXCHANGE; AIR IONIZATION nor PHONOPHORESIS, none of which requires current. Iontophoreses
D008297 Male Males
D004058 Diffusion The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT. Diffusions
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000279 Administration, Cutaneous The application of suitable drug dosage forms to the skin for either local or systemic effects. Cutaneous Drug Administration,Dermal Drug Administration,Drug Administration, Dermal,Percutaneous Administration,Skin Drug Administration,Transcutaneous Administration,Transdermal Administration,Administration, Dermal,Administration, Transcutaneous,Administration, Transdermal,Cutaneous Administration,Cutaneous Administration, Drug,Dermal Administration,Drug Administration, Cutaneous,Skin Administration, Drug,Administration, Cutaneous Drug,Administration, Dermal Drug,Administration, Percutaneous,Administrations, Cutaneous,Administrations, Cutaneous Drug,Administrations, Dermal,Administrations, Dermal Drug,Administrations, Percutaneous,Administrations, Transcutaneous,Administrations, Transdermal,Cutaneous Administrations,Cutaneous Administrations, Drug,Cutaneous Drug Administrations,Dermal Administrations,Dermal Drug Administrations,Drug Administrations, Cutaneous,Drug Administrations, Dermal,Drug Skin Administrations,Percutaneous Administrations,Skin Administrations, Drug,Skin Drug Administrations,Transcutaneous Administrations,Transdermal Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012867 Skin The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D012869 Skin Absorption Uptake of substances through the SKIN. Absorption, Skin,Intracutaneous Absorption,Intradermal Absorption,Percutaneous Absorption,Transcutaneous Absorption,Transdermal Absorption,Absorption, Intracutaneous,Absorption, Intradermal,Absorption, Percutaneous,Absorption, Transcutaneous,Absorption, Transdermal,Absorptions, Intracutaneous,Absorptions, Intradermal,Absorptions, Percutaneous,Absorptions, Skin,Absorptions, Transcutaneous,Absorptions, Transdermal,Intracutaneous Absorptions,Intradermal Absorptions,Percutaneous Absorptions,Skin Absorptions,Transcutaneous Absorptions,Transdermal Absorptions
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D017325 Hepatitis B Vaccines Vaccines or candidate vaccines containing inactivated hepatitis B or some of its component antigens and designed to prevent hepatitis B. Some vaccines may be recombinantly produced. Hepatitis B Vaccine,Vaccine, Hepatitis B,Vaccines, Hepatitis B

Related Publications

Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Transdermal iontophoresis of hydromorphone across hairless rat skin in vitro.
January 2009, Pharmaceutical development and technology,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Transdermal iontophoresis and solute penetration across excised human skin.
November 1995, Journal of pharmaceutical sciences,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
The pretreatment effect of chemical skin penetration enhancers in transdermal drug delivery using iontophoresis.
January 1999, Skin pharmacology and applied skin physiology,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Effect of penetration enhancers on isosorbide dinitrate penetration through rat skin from a transdermal therapeutic system.
April 2000, International journal of pharmaceutics,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
In vitro acyclovir distribution in human skin layers after transdermal iontophoresis.
January 1998, Journal of controlled release : official journal of the Controlled Release Society,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Effect of penetration enhancers on the transdermal delivery of bupranolol through rat skin.
January 2005, Drug delivery,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Penetration of ammonium perfluorooctanoate through rat and human skin in vitro.
January 2005, Drug and chemical toxicology,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Effect of penetration enhancers on in vitro percutaneous penetration of nimesulide through rat skin.
January 2004, Die Pharmazie,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
Regional differences in transdermal penetration of fentanyl through equine skin.
April 2007, Research in veterinary science,
Ting Xu, and Yue-Hong Xu, and Min-Yan Wei, and Li-He Deng, and Chuan-Bin Wu
In vitro percutaneous penetration through hairless rat skin: influence of temperature, vehicle and penetration enhancers.
November 1998, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V,
Copied contents to your clipboard!