Arabidopsis LOS5/ABA3 overexpression in transgenic tobacco (Nicotiana tabacum cv. Xanthi-nc) results in enhanced drought tolerance. 2011

Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
State Key Laboratory of Plant Physiology and Biochemistry, Department of Agronomy, Centre of Crop Chemical Control, College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan Xi Lu, Haidian District, Beijing, PR China.

Drought is a major environmental stress factor that affects growth and development of plants. Abscisic acid (ABA), osmotically active compounds, and synthesis of specific proteins, such as proteins that scavenge oxygen radicals, are crucial for plants to adapt to water deficit. LOS5/ABA3 (LOS5) encodes molybdenum-cofactor sulfurase, which is a key regulator of ABA biosynthesis. We overexpressed LOS5 in tobacco using Agrobacterium-mediated transformation. Detached leaves of LOS5-overexpressing seedlings showed lower transpirational water loss than that of nontransgenic seedlings in the same period under normal conditions. When subjected to water-deficit stress, transgenic plants showed less wilting, maintained higher water content and better cellular membrane integrity, accumulated higher quantities of ABA and proline, and exhibited higher activities of antioxidant enzymes, i.e., superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, as compared with control plants. Furthermore, LOS5-overexpressing plants treated with 30% polyethylene glycol showed similar performance in cellular membrane protection, ABA and proline accumulation, and activities of catalase and peroxidase to those under drought stress. Thus, overexpression of LOS5 in transgenic tobacco can enhance drought tolerance.

UI MeSH Term Description Entries
D011392 Proline A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons. L-Proline,L Proline
D004573 Electrolytes Substances that dissociate into two or more ions, to some extent, in water. Solutions of electrolytes thus conduct an electric current and can be decomposed by it (ELECTROLYSIS). (Grant & Hackh's Chemical Dictionary, 5th ed) Electrolyte
D000040 Abscisic Acid Abscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits. 2,4-Pentadienoic acid, 5-(1-hydroxy-2,6,6-trimethyl-4-oxo-2-cyclohexen-1-yl)-3-methyl-, (S-(Z,E))-,Abscisic Acid Monoammonium Salt, (R)-Isomer,Abscisic Acid, (+,-)-Isomer,Abscisic Acid, (E,E)-(+-)-Isomer,Abscisic Acid, (E,Z)-(+,-)-Isomer,Abscisic Acid, (R)-Isomer,Abscisic Acid, (Z,E)-Isomer,Abscissic Acid,Abscissins
D000222 Adaptation, Physiological The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT. Adaptation, Physiologic,Adaptations, Physiologic,Adaptations, Physiological,Adaptive Plasticity,Phenotypic Plasticity,Physiological Adaptation,Physiologic Adaptation,Physiologic Adaptations,Physiological Adaptations,Plasticity, Adaptive,Plasticity, Phenotypic
D000975 Antioxidants Naturally occurring or synthetic substances that inhibit or retard oxidation reactions. They counteract the damaging effects of oxidation in animal tissues. Anti-Oxidant,Antioxidant,Antioxidant Activity,Endogenous Antioxidant,Endogenous Antioxidants,Anti-Oxidant Effect,Anti-Oxidant Effects,Anti-Oxidants,Antioxidant Effect,Antioxidant Effects,Activity, Antioxidant,Anti Oxidant,Anti Oxidant Effect,Anti Oxidant Effects,Anti Oxidants,Antioxidant, Endogenous,Antioxidants, Endogenous
D013466 Sulfurtransferases Enzymes which transfer sulfur atoms to various acceptor molecules. EC 2.8.1. Sulfurtransferase
D013481 Superoxides Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides. Superoxide Radical,Superoxide,Superoxide Anion
D014026 Nicotiana A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING. Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants
D015227 Lipid Peroxidation Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor. Lipid Peroxidations,Peroxidation, Lipid,Peroxidations, Lipid
D017360 Arabidopsis A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. Arabidopsis thaliana,Cress, Mouse-ear,A. thaliana,A. thalianas,Arabidopses,Arabidopsis thalianas,Cress, Mouse ear,Cresses, Mouse-ear,Mouse-ear Cress,Mouse-ear Cresses,thaliana, A.,thaliana, Arabidopsis,thalianas, A.

Related Publications

Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
July 1998, Phytopathology,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
March 2007, Plant cell reports,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
March 1988, Plant molecular biology,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
December 2013, Molecular biology reports,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
September 2003, Archives of virology,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
March 2022, Plant biology (Stuttgart, Germany),
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
January 2012, Jundishapur journal of natural pharmaceutical products,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
October 2014, Plant physiology and biochemistry : PPB,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
February 2014, Plant physiology and biochemistry : PPB,
Yuesen Yue, and Mingcai Zhang, and Jiachang Zhang, and Liusheng Duan, and Zhaohu Li
October 2019, Plant science : an international journal of experimental plant biology,
Copied contents to your clipboard!