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Dengue viruses (DENV) cause countless human deaths each year, whilst West Nile virus (WNV) has re-emerged as an important human pathogen. There are currently no WNV or DENV vaccines licensed for human use, yet vaccines exist against other flaviviruses. To investigate flavivirus cross-reactivity, sera from a human cohort with a history of vaccination against tick-borne encephalitis virus (TBEV), Japanese encephalitis virus (JEV) and yellow fever virus (YFV) were tested for antibodies by plaque reduction neutralization test. Neutralization of louping ill virus (LIV) occurred, but no significant neutralization of Murray Valley encephalitis virus was observed. Sera from some individuals vaccinated against TBEV and JEV neutralized WNV, which was enhanced by YFV vaccination in some recipients. Similarly, some individuals neutralized DENV-2, but this was not significantly influenced by YFV vaccination. Antigenic cartography techniques were used to generate a geometric illustration of the neutralization titres of selected sera against WNV, TBEV, JEV, LIV, YFV and DENV-2. This demonstrated the individual variation in antibody responses. Most sera had detectable titres against LIV and some had titres against WNV and DENV-2. Generally, LIV titres were similar to titres against TBEV, confirming the close antigenic relationship between TBEV and LIV. JEV was also antigenically closer to TBEV than WNV, using these sera. The use of sera from individuals vaccinated against multiple pathogens is unique relative to previous applications of antigenic cartography techniques. It is evident from these data that notable differences exist between amino acid sequence identity and mapped antigenic relationships within the family Flaviviridae.
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
November 2018,
Cell host & microbe,
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
May 2020,
Journal of virology,
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
March 2002,
Medical microbiology and immunology,
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
December 2019,
Diagnostics (Basel, Switzerland),
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
October 1987,
The Journal of general virology,
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
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Research in immunology,
Karen L Mansfield, and
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Li Li, and
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Derek J Smith, and
Sareen E Galbraith, and
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Toxins,
Karen L Mansfield, and
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Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
September 2023,
International journal of molecular sciences,
Karen L Mansfield, and
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Sareen E Galbraith, and
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Anthony R Fooks
August 2017,
Transplant infectious disease : an official journal of the Transplantation Society,
Karen L Mansfield, and
Daniel L Horton, and
Nicholas Johnson, and
Li Li, and
Alan D T Barrett, and
Derek J Smith, and
Sareen E Galbraith, and
Tom Solomon, and
Anthony R Fooks
August 2017,
Transplant infectious disease : an official journal of the Transplantation Society,
