Hypoxia-dependent inhibition of tumor cell susceptibility to CTL-mediated lysis involves NANOG induction in target cells. 2011

Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
Institut Gustave Roussy, INSERM Unité 753, 94800 Villejuif, France.

Hypoxia is a major feature of the solid tumor microenvironment and is known to be associated with tumor progression and poor clinical outcome. Recently, we reported that hypoxia protects human non-small cell lung tumor cells from specific lysis by stabilizing hypoxia-inducible factor-1α and inducing STAT3 phosphorylation. In this study, we show that NANOG, a transcription factor associated with stem cell self renewal, is a new mediator of hypoxia-induced resistance to specific lysis. Our data indicate that under hypoxic conditions, NANOG is induced at both transcriptional and translational levels. Knockdown of the NANOG gene in hypoxic tumor cells is able to significantly attenuate hypoxia-induced tumor resistance to CTL-dependent killing. Such knockdown correlates with an increase of target cell death and an inhibition of hypoxia-induced delay of DNA replication in these cells. Interestingly, NANOG depletion results in inhibition of STAT3 phosphorylation and nuclear translocation. To our knowledge, this study is the first to show that hypoxia-induced NANOG plays a critical role in tumor cell response to hypoxia and promotes tumor cell resistance to Ag-specific lysis.

UI MeSH Term Description Entries
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000071317 Nanog Homeobox Protein A homeodomain protein and transcription regulator that functions in BLASTOCYST INNER CELL MASS and EMBRYONIC STEM CELL proliferation and CELL SELF RENEWAL. It confers pluripotency on embryonic stem cells and prevents their differentiation towards extraembryonic ENDODERM and trophectoderm (TROPHOBLAST) CELL LINEAGES. Nanog Homeobox Transcription Factor,Nanog Protein,Homeobox Protein, Nanog
D013602 T-Lymphocytes, Cytotoxic Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2. Cell-Mediated Lympholytic Cells,Cytotoxic T Cells,Cytotoxic T Lymphocyte,Cytotoxic T-Lymphocytes,TC1 Cell,TC1 Cells,TC2 Cell,TC2 Cells,Cell Mediated Lympholytic Cells,Cell, Cell-Mediated Lympholytic,Cell, TC1,Cell, TC2,Cell-Mediated Lympholytic Cell,Cytotoxic T Cell,Cytotoxic T Lymphocytes,Cytotoxic T-Lymphocyte,Lymphocyte, Cytotoxic T,Lympholytic Cell, Cell-Mediated,Lympholytic Cells, Cell-Mediated,T Cell, Cytotoxic,T Lymphocyte, Cytotoxic,T Lymphocytes, Cytotoxic,T-Lymphocyte, Cytotoxic
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D015687 Cell Hypoxia A condition of decreased oxygen content at the cellular level. Anoxia, Cellular,Cell Anoxia,Hypoxia, Cellular,Anoxia, Cell,Anoxias, Cell,Anoxias, Cellular,Cell Anoxias,Cell Hypoxias,Cellular Anoxia,Cellular Anoxias,Cellular Hypoxia,Cellular Hypoxias,Hypoxia, Cell,Hypoxias, Cell,Hypoxias, Cellular
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions

Related Publications

Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
January 2005, Journal of immunology (Baltimore, Md. : 1950),
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
June 1998, Journal of cellular biochemistry,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
April 1975, European journal of immunology,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
November 1989, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
October 2013, Proceedings of the National Academy of Sciences of the United States of America,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
April 2016, PLoS pathogens,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
May 2010, Journal of immunotherapy (Hagerstown, Md. : 1997),
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
May 1993, Journal of immunotherapy with emphasis on tumor immunology : official journal of the Society for Biological Therapy,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
April 1993, International journal of cancer,
Meriem Hasmim, and Muhammad Zaeem Noman, and Jessica Lauriol, and Houssem Benlalam, and Aude Mallavialle, and Filippo Rosselli, and Fathia Mami-Chouaib, and Catherine Alcaide-Loridan, and Salem Chouaib
April 1985, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!