Fast analysis of amino acids in wine by capillary electrophoresis with laser-induced fluorescence detection. 2011

Roberto Mandrioli, and Emanuele Morganti, and Laura Mercolini, and Ernst Kenndler, and Maria A Raggi
Pharmaco-Toxicological Analysis Laboratory, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Bologna, Italy.

A fast analytical method has been developed for the determination of nine amino acids, together with serotonin, in wine samples of different origin and vintage. The method is based on capillary electrophoresis coupled to laser-induced fluorescence detection. Separation was obtained by using a fused-silica capillary (75 μm id, 74.0 cm total length, 60.0 cm length to detector) and a background electrolyte composed of carbonate buffer (20 mM, pH 9.2), applying a 20 kV voltage. Direct hydrodynamic injection of wine samples was made after an original microwave-assisted derivatisation step with 5-(4,6-dichlorotriazinyl)aminofluorescein. Fluorescence was induced by an Ar-Ion laser, exciting at 488 nm. Good linearity (r(2) >0.9990) was obtained for all considered analytes and sensitivity was also good, with limits of detection in the 7-50 ng/mL range. The method was successfully applied for the analysis of commercial Italian wines and thus seems to be suitable for the determination of the relevant amino acids and serotonin, providing good results in terms of accuracy and precision, together with the advantage of a very fast, microwave-assisted derivatisation procedure. Future applications of the method are planned to check for wine adulterations and commercial frauds.

UI MeSH Term Description Entries
D000596 Amino Acids Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins. Amino Acid,Acid, Amino,Acids, Amino
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D014920 Wine Fermented juice of fresh grapes or of other fruit or plant products used as a beverage. Wines
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016014 Linear Models Statistical models in which the value of a parameter for a given value of a factor is assumed to be equal to a + bx, where a and b are constants. The models predict a linear regression. Linear Regression,Log-Linear Models,Models, Linear,Linear Model,Linear Regressions,Log Linear Models,Log-Linear Model,Model, Linear,Model, Log-Linear,Models, Log-Linear,Regression, Linear,Regressions, Linear
D054020 Lasers, Gas Lasers in which a gas lasing medium is stimulated to emit light by an electric current or high-frequency oscillator. Argon Ion Lasers,CO2 Lasers,Carbon Dioxide Lasers,Copper Vapor Lasers,Gas Lasers,Gold Vapor Lasers,Helium Lasers,Helium Neon Gas Lasers,Metal Vapor Lasers,Nitrogen Lasers,Xenon Ion Lasers,HeNe Lasers,Lasers, Argon Ion,Lasers, CO2,Lasers, Carbon Dioxide,Lasers, Copper Vapor,Lasers, Gold Vapor,Lasers, Helium,Lasers, Helium Neon Gas,Lasers, Metal Vapor,Lasers, Nitrogen,Lasers, Xenon Ion,Argon Ion Laser,CO2 Laser,Carbon Dioxide Laser,Copper Vapor Laser,Dioxide Laser, Carbon,Dioxide Lasers, Carbon,Gas Laser,Gold Vapor Laser,HeNe Laser,Helium Laser,Ion Laser, Argon,Ion Laser, Xenon,Ion Lasers, Argon,Ion Lasers, Xenon,Laser, Argon Ion,Laser, CO2,Laser, Carbon Dioxide,Laser, Copper Vapor,Laser, Gas,Laser, Gold Vapor,Laser, HeNe,Laser, Helium,Laser, Metal Vapor,Laser, Nitrogen,Laser, Xenon Ion,Lasers, HeNe,Metal Vapor Laser,Nitrogen Laser,Vapor Laser, Copper,Vapor Laser, Gold,Vapor Laser, Metal,Vapor Lasers, Copper,Vapor Lasers, Gold,Vapor Lasers, Metal,Xenon Ion Laser
D057230 Limit of Detection Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure. Limits of Detection,Detection Limit,Detection Limits
D019075 Electrophoresis, Capillary A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992) Capillary Zone Electrophoresis,Capillary Electrophoreses,Capillary Electrophoresis,Capillary Zone Electrophoreses,Electrophoreses, Capillary,Electrophoreses, Capillary Zone,Electrophoresis, Capillary Zone,Zone Electrophoreses, Capillary,Zone Electrophoresis, Capillary

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