BIOMAT Database Logo BIOMAT Database Logo

Detection of Borrelia burgdorferi using the polymerase chain reaction. 1990

D C Malloy, and R K Nauman, and H Paxton
Maryland Medical Laboratory, Inc., Baltimore 21227.

Segments of the ospA gene of Borrelia burgdorferi were amplified by the polymerase chain reaction (PCR). Oligonucleotide primers used in the reaction flank a 309-base-pair segment within the ospA gene. After 35 cycles of amplification, the product could be detected by agarose gel electrophoresis or dot hybridization with a 32P-labeled probe. This segment was amplified in all strains of B. burgdorferi tested, but it was not detected in other bacterial species. An additional primer pair which has a broad specificity for conserved 16S rRNA sequences that are present in eubacteria amplified a 215-base-pair fragment in all organisms tested. The sensitivity of PCR for the detection of B. burgdorferi in clinical samples was evaluated by seeding blood and urine specimens with B. burgdorferi and subjecting them to amplification. We were able to detect 10 organisms per ml of blood or urine, using PCR with dot hybridization detection. DNA extraction is not required for sample preparation. Blood and urine specimens were obtained from canines with clinical and serologic evidence of Lyme disease and subjected to PCR analysis. Of 17 clinical specimens from 15 animals, one blood specimen showed reactivity in the PCR.

UI MeSH Term Description Entries
D008193 Lyme Disease An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut. Lyme Borreliosis,B. burgdorferi Infection,Borrelia burgdorferi Infection,Lyme Arthritis,Arthritis, Lyme,B. burgdorferi Infections,Borrelia burgdorferi Infections,Borreliosis, Lyme,Disease, Lyme
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001425 Bacterial Outer Membrane Proteins Proteins isolated from the outer membrane of Gram-negative bacteria. OMP Proteins,Outer Membrane Proteins, Bacterial,Outer Membrane Lipoproteins, Bacterial
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot

Related Publications

D C Malloy, and R K Nauman, and H Paxton
Detection of Borrelia burgdorferi DNA by the polymerase chain reaction.
February 1990, Molecular and cellular probes,
D C Malloy, and R K Nauman, and H Paxton
[Vulvar pseudolymphoma: detection of infection by Borrelia burgdorferi using polymerase chain reaction].
January 1998, Gynakologisch-geburtshilfliche Rundschau,
D C Malloy, and R K Nauman, and H Paxton
Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay.
June 1991, Research in microbiology,
D C Malloy, and R K Nauman, and H Paxton
Detection of Borrelia burgdorferi in human blood and urine using the polymerase chain reaction.
January 1992, Pathobiology : journal of immunopathology, molecular and cellular biology,
D C Malloy, and R K Nauman, and H Paxton
Rapid detection of Borrelia burgdorferi strains by nested polymerase chain reaction.
March 2009, Pakistan journal of biological sciences : PJBS,
D C Malloy, and R K Nauman, and H Paxton
Detection of Borrelia burgdorferi in cerebrospinal fluid by the polymerase chain reaction.
August 1991, Journal of medical microbiology,
D C Malloy, and R K Nauman, and H Paxton
Detection and genotyping of Borrelia burgdorferi sensu lato by polymerase chain reaction.
March 2000, Croatian medical journal,
D C Malloy, and R K Nauman, and H Paxton
[Polymerase chain reaction in the demonstration of Borrelia burgdorferi DNA].
November 1990, Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete,
D C Malloy, and R K Nauman, and H Paxton
Polymerase chain reaction amplification of culture supernatants for rapid detection of Borrelia burgdorferi.
November 1993, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
D C Malloy, and R K Nauman, and H Paxton
The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids.
May 1993, Arthritis and rheumatism,
Copied contents to your clipboard!