BIOMAT Database Logo BIOMAT Database Logo

Adrenaline and dopamine in porcine livers after cold preservation with University of Wisconsin histidine-tryptophan-ketoglutarate, prolactin-modified histidine-tryptophan-ketoglutarate solutions. 2011

G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland. gregorydr@wp.pl

BACKGROUND Hepatic ischemia-reperfusion injury remains a significant factor influencing early liver graft function. The aim of this study was to assess the impact on hepatic ischemia as reflected by catecholamine concentrations of different methods of organ preservation. METHODS Catecholamine levels were measured in 24 (n=6/group) pig livers, which underwent 30-minute warm ischemia followed by 30-minute perfusion and subsequent cold storage for 12 hours. For perfusion and preservation, we used University of Wisconsin (UW), histidine-tryptophan-ketoglutarate (HTK), HTK-modified with prolactin (PRL) or Ringer's solutions. Dopamine (DO) and adrenaline (ADR) concentrations in liver venous effluents were assayed using a radioimmunological method after 30 minutes of perfusion and following 12 hours of preservation. RESULTS DO and ADR levels were higher after 12 hours preservation compared to 30 minutes of perfusion. HTK produced an increase of over 100%. Addition of PRL (20 IU/L) did not affect DO and ADR levels after 30 minutes of perfusion, but significantly decreased their concentrations at 12 hours of preservation. After UW perfusion and preservation, we observed a 10% increase in catecholamine levels as compared with postperfusion values. Preservation with Ringer's solution demonstrated significantly higher DO and ADR levels compared with other solutions. CONCLUSIONS Catecholamines are present in the liver after 30 minute of perfusion and 12 hours of cold storage. The increased levels after 12 hours of preservation may be due to their release from intracellular spaces (as a controlled process or as a result of necrosis). It may play a crucial role in reperfusion injury, which, in turn, may explain the mechanism of no-reflow phenomenon.

UI MeSH Term Description Entries
D007328 Insulin A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1). Iletin,Insulin A Chain,Insulin B Chain,Insulin, Regular,Novolin,Sodium Insulin,Soluble Insulin,Chain, Insulin B,Insulin, Sodium,Insulin, Soluble,Regular Insulin
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008353 Mannitol A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity. (L)-Mannitol,Osmitrol,Osmofundin
D009926 Organ Preservation The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Organ Preservations,Preservation, Organ,Preservations, Organ
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D011189 Potassium Chloride A white crystal or crystalline powder used in BUFFERS; FERTILIZERS; and EXPLOSIVES. It can be used to replenish ELECTROLYTES and restore WATER-ELECTROLYTE BALANCE in treating HYPOKALEMIA. Slow-K,Chloride, Potassium
D011343 Procaine A local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1016). Anuject,Geriocaine,Gerokit,Hewedolor-Procain,Lophakomp-Procain N,Novocain,Novocaine,Procain Braun,Procain Jenapharm,Procain Rödler,Procain Steigerwald,Procain curasan,Procaina Serra,Procaine Hydrochloride,Pröcaine chlorhydrate Lavoisier,Röwo Procain,procain-loges,Hydrochloride, Procaine
D011388 Prolactin A lactogenic hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). It is a polypeptide of approximately 23 kD. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin receptors are present in the mammary gland, hypothalamus, liver, ovary, testis, and prostate. Lactogenic Hormone, Pituitary,Mammotropic Hormone, Pituitary,Mammotropin,PRL (Prolactin),Hormone, Pituitary Lactogenic,Hormone, Pituitary Mammotropic,Pituitary Lactogenic Hormone,Pituitary Mammotropic Hormone
D011887 Raffinose A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal. Gossypose,Melitose,Melitriose
D003080 Cold Temperature An absence of warmth or heat or a temperature notably below an accustomed norm. Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold

Related Publications

G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Comparison of Histidine-Tryptophan-Ketoglutarate and University of Wisconsin preservation solutions in pediatric liver transplantation.
June 2018, Pediatric transplantation,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Cold preservation of the porcine pancreas with histidine-tryptophan-ketoglutarate solution.
November 1998, Transplantation,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
P-selectin expression in cold preserved kidneys in University of Wisconsin and histidine-tryptophan-ketoglutarate solutions.
July 2011, The Journal of surgical research,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Experience with hystidine tryptophan ketoglutarate versus University Wisconsin preservation solutions in transplantation.
January 2003, International surgery,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Comparison of histidine-tryptophan-ketoglutarate and University of Wisconsin preservation in renal transplantation.
March 2008, American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Comparison of histidine-tryptophan-ketoglutarate and University of Wisconsin preservation in renal transplantation.
September 2008, American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Experimental liver preservation with Celsior: a novel alternative to University of Wisconsin and histidine-tryptophan-alpha-ketoglutarate solutions?
January 2000, European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Comparison of histidine-tryptophan ketoglutarate solution and University of Wisconsin solution in prolonged cold preservation of kidney allografts.
February 2006, Transplantation,
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Glutathione, lactobionate, and histidine: cryptic inhibitors of matrix metalloproteinases contained in University of Wisconsin and histidine/tryptophan/ketoglutarate liver preservation solutions.
May 2000, Hepatology (Baltimore, Md.),
G Budziński, and A Suszka-Świtek, and R Dec, and A Caban, and W Wystrychowski, and G Oczkowicz, and M Heitzman, and B Dolińska, and F Ryszka, and L Cierpka
Randomized clinical assay for hepatic grafts preservation with University of Wisconsin or histidine-tryptophan-ketoglutarate solutions in liver transplantation.
January 2006, Transplantation proceedings,
Copied contents to your clipboard!