Serum antibodies protect against intraperitoneal challenge with enterotoxigenic Escherichia coli. 2011

Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
Department of Immunology & Infectious Diseases, Montana State University, Bozeman, MT 59717-3610, USA.

To assess whether anticolonization factor antigen I (CFA/I) fimbriae antibodies (Abs) from enterotoxigenic Escherichia coli (ETEC) can protect against various routes of challenge, BALB/c mice were immunized with a live attenuated Salmonella vaccine vector expressing CFA/I fimbriae. Vaccinated mice elicited elevated systemic IgG and mucosal IgA Abs, unlike mice immunized with the empty Salmonella vector. Mice were challenged with wild-type ETEC by the oral, intranasal (i.n.), and intraperitoneal (i.p.) routes. Naïve mice did not succumb to oral challenge, but did to i.n. challenge, as did immunized mice; however, vaccinated mice were protected against i.p. ETEC challenge. Two intramuscular (i.m.) immunizations with CFA/I fimbriae without adjuvant conferred 100% protection against i.p. ETEC challenge, while a single 30 μg dose conferred 88% protection. Bactericidal assays showed that ETEC is highly sensitive to anti-CFA/I sera. These results suggest that parenteral immunization with purified CFA/I fimbriae can induce protective Abs and may represent an alternative method to elicit protective Abs for passive immunity to ETEC.

UI MeSH Term Description Entries
D007070 Immunoglobulin A Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions. IgA,IgA Antibody,IgA1,IgA2,Antibody, IgA
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D007274 Injections, Intraperitoneal Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall. Intraperitoneal Injections,Injection, Intraperitoneal,Intraperitoneal Injection
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D001770 Blood Bactericidal Activity The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST. Activities, Blood Bactericidal,Activity, Blood Bactericidal,Bactericidal Activities, Blood,Bactericidal Activity, Blood,Blood Bactericidal Activities
D004927 Escherichia coli Infections Infections with bacteria of the species ESCHERICHIA COLI. E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D005260 Female Females
D000281 Administration, Intranasal Delivery of medications through the nasal mucosa. Drug Administration, Intranasal,Administration, Intranasal Drug,Administration, Nasal,Intranasal Administration,Intranasal Drug Administration,Administrations, Intranasal,Administrations, Intranasal Drug,Administrations, Nasal,Drug Administrations, Intranasal,Intranasal Administrations,Intranasal Drug Administrations,Nasal Administration,Nasal Administrations
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations

Related Publications

Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
March 1998, The Journal of infectious diseases,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
February 1988, Infection and immunity,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
May 1988, The New England journal of medicine,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
September 2014, Applied and environmental microbiology,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
August 2008, Expert review of vaccines,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
May 1986, Infection and immunity,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
January 2022, Frontiers in immunology,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
January 1988, Gynecologic and obstetric investigation,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
November 2020, Gut microbes,
Xinghong Yang, and Theresa Thornburg, and Kathryn Holderness, and Zhiyong Suo, and Ling Cao, and Timothy Lim, and Recep Avci, and David W Pascual
January 1986, Progress in veterinary microbiology and immunology,
Copied contents to your clipboard!