In vivo optical imaging of membrane-type matrix metalloproteinase (MT-MMP) activity. 2011

Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, United States.

Herein we demonstrate for the first time that a fluorogenic probe can be used as an in vivo imaging agent for visualizing activities of membrane-tethered, membrane-type matrix metalloproteinases (MT-MMPs). An MT-MMP fluorogenic probe that consisted of an MT1-MMP (MMP-14) substrate and near-infrared (NIR) dye-quencher pair exhibited rapid, efficient boosts in fluorescence upon cleavage by MT1-MMP in tumor-bearing mice. In particular, unlike similar fluorogenic probes designed to target extracellular, soluble-type MMPs (EC-MMPs)--which can be cleared from the bloodstream after activation--the fluorescence signals activated by MT1-MMP enable clear visualization of MT1-MMP-positive tumors in animal models for up to 24 h. The results indicate that a simple form of a fluorogenic probe that is less effective in EC-MMP imaging is an effective probe for imaging MT-MMP activities in vivo. These findings can be widely applied to designing probes and to applications targeting various membrane-anchored proteases in vivo.

UI MeSH Term Description Entries
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D053504 Matrix Metalloproteinases, Membrane-Associated Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION. Membrane-Type Matrix Metalloproteinase,MT-MMP Enzymes,Matrix Metalloproteinase, Membrane-Type,Membrane-Type Matrix Metalloproteinases,MT MMP Enzymes,Matrix Metalloproteinase, Membrane Type,Matrix Metalloproteinases, Membrane Associated,Matrix Metalloproteinases, Membrane-Type,Membrane Type Matrix Metalloproteinase,Membrane Type Matrix Metalloproteinases,Membrane-Associated Matrix Metalloproteinases,Metalloproteinase, Membrane-Type Matrix,Metalloproteinases, Membrane-Associated Matrix,Metalloproteinases, Membrane-Type Matrix
D057054 Molecular Imaging The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY. Imaging, Molecular

Related Publications

Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
January 2003, Current topics in developmental biology,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
July 1995, Nihon rinsho. Japanese journal of clinical medicine,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
December 1999, The American journal of physiology,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
April 2003, Journal of periodontology,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
October 2001, Tissue & cell,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
September 1995, The Journal of biological chemistry,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
February 2002, Journal of dermatological science,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
July 2016, Scientific reports,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
March 1997, Journal of cell science,
Lei Zhu, and Fan Zhang, and Ying Ma, and Gang Liu, and Kwangmeyung Kim, and Xuexun Fang, and Seulki Lee, and Xiaoyuan Chen
February 1998, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Copied contents to your clipboard!