Electrochemical biosensor based on graphene oxide-Au nanoclusters composites for L-cysteine analysis. 2012

Shenguang Ge, and Mei Yan, and Juanjuan Lu, and Meng Zhang, and Feng Yu, and Jinghua Yu, and Xianrang Song, and Shilin Yu
Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.

In this paper, a linker-free connected graphene oxide/Au nanocluster (GO-Au NCs) composite was prepared under sonication through electrostatic interactions, and characterized by transmission electron microscope (TEM), atomic force microscope (AFM), ultraviolet-visible (UV-vis) and FT-IR spectrum. The morphological and structural characterizations evidence that the Au NCs can be efficiently decorated on the GO. The electrochemical investigations indicated that GO-Au NCs composite has an important role in the electrocatalytic activity towards the oxidation of L-cysteine (CySH). The GO-Au NCs composite modified electrode shows a large determination range from 0.05 to 20.0 μmol/L, a remarkably low detection limit is 0.02 μmol/L and low oxidation potential (+0.387). It was found that metal ions, carbohydrates, nucleotide acids and amino acids had no distinct effect on the determination of L-cysteine. In addition, the sensor has some important advantages such as simple preparation, fast response, good stability and high reproducibility. The direct determination of free reduced and total CySH in human urine samples has been successfully carried out without the assistance of any separation techniques.

UI MeSH Term Description Entries
D010087 Oxides Binary compounds of oxygen containing the anion O(2-). The anion combines with metals to form alkaline oxides and non-metals to form acidic oxides. Oxide
D003217 Conductometry Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed) Titration, Conductometric,Conductometric Titration,Conductometric Titrations,Titrations, Conductometric
D003545 Cysteine A thiol-containing non-essential amino acid that is oxidized to form CYSTINE. Cysteine Hydrochloride,Half-Cystine,L-Cysteine,Zinc Cysteinate,Half Cystine,L Cysteine
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D006108 Graphite An allotropic form of carbon that is used in pencils, as a lubricant, and in matches and explosives. It is obtained by mining and its dust can cause lung irritation. Graphene
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D049329 Nanostructures Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES. Nanomaterials,Nanostructured Materials,Material, Nanostructured,Materials, Nanostructured,Nanomaterial,Nanostructure,Nanostructured Material
D019544 Equipment Failure Analysis The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices. Materials Failure Analysis,Prosthesis Failure Analysis,Analysis, Equipment Failure,Analysis, Materials Failure,Analysis, Prosthesis Failure,Analyses, Equipment Failure,Analyses, Materials Failure,Analyses, Prosthesis Failure,Equipment Failure Analyses,Failure Analyses, Equipment,Failure Analyses, Materials,Failure Analyses, Prosthesis,Failure Analysis, Equipment,Failure Analysis, Materials,Failure Analysis, Prosthesis,Materials Failure Analyses,Prosthesis Failure Analyses
D036103 Nanotechnology The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller. Nanotechnologies

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