OBJECTIVE The present study was undertaken to determine the prevalence of respiratory, gastrointestinal and other pathogens in 100 Human Immunodeficiency Virus (HIV) seropositive patients. METHODS This study was carried out on randomly selected 100 HIV seropositive patients from S. S. G. Hospital during the period from Jan 2006 to Jan 2007. METHODS Sputum samples, stool samples and oral swabs were collected from all the patients and cerebrospinal fluid (CSF) was collected from symptomatic patients and processed as per the standard protocol. Sputum samples were examined by microscopy and cultured for bacterial respiratory pathogens. Stool samples were concentrated and examined by microscopy for enteric parasites. Oral swabs and CSF were also examined microscopically and cultured for fungal pathogens. CSF was also examined for bacterial pathogens. RESULTS A total of 101 pathogens were detected in 60 patients. More than one pathogen was observed in 30 patients. Candida was the commonest isolate (32.67%), followed by Mycobacterium tuberculosis (22.71%) and Cryptosporidium parvum (19.8%). CONCLUSIONS Since opportunistic infections are a major cause of mortality and morbidity in HIV seropositive patients, an early diagnosis and effective treatment are required to tackle them. The type of pathogens infecting HIV patients varies from region to region, and therefore such patients should be constantly screened for these pathogens.