Biomaterial Database

Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission. 2011

Arijit Kumar De, and Debjit Roy, and Debabrata Goswami

Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.

Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.

UI	MeSH Term	Description	Entries
D007211	Indoles	Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D007834	Lasers	An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.	Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014966	Xanthenes	Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.	Xanthene
D055620	Optical Phenomena	LIGHT, its processes and properties, and the characteristics of materials interacting with it.	Optical Concepts,Optical Processes,Optical Phenomenon,Optical Process,Concept, Optical,Concepts, Optical,Optical Concept,Phenomena, Optical,Phenomenon, Optical,Process, Optical,Processes, Optical
D036641	Microscopy, Fluorescence, Multiphoton	Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.	Fluorescence Microscopy, Multiphoton,Multiphoton Fluorescence Microscopy,Multiphoton Excitation Microscopy,Excitation Microscopies, Multiphoton,Excitation Microscopy, Multiphoton,Microscopies, Multiphoton Excitation,Microscopy, Multiphoton Excitation,Microscopy, Multiphoton Fluorescence,Multiphoton Excitation Microscopies