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Hydrolysis of synthetic chromogenic substrates by HIV-1 and HIV-2 proteinases. 1990
L H Phylip, and
A D Richards, and
J Kay, and
J Kovalinka, and
P Strop, and
I Blaha, and
J Velek, and
V Kostka, and
A J Ritchie, and
A V Broadhurst
Department of Biochemistry, University of Wales College, Cardiff, U.K.
Kinetic constants (Km,Kcat) are derived for the hydrolysis of a number of chromogenic peptide substrates by the aspartic proteinase from HIV-2. The effect of systematic replacement of the P2 residue on substrate hydrolysis by HIV-1 and HIV-2 proteinases is examined.
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