Sexing single bovine blastomeres using TSPY gene amplification. 2011

M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.

The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same embryo were concordant in all cases. All blastomeres (16/16) from eight embryos produced with sexed sperm (specific for production of male embryos) were TSPY-positive. We conclude that TSPY is a good male-specific marker, the usefulness of which is probably enhanced by the high copy number. Other methods that are less time-consuming, such as real-time PCR, could be improved with the use of the TSPY gene sequences to generate primers and/or probes. This is the first report to demonstrate the applicability of the TSPY gene for sexing single cells in cattle.

UI MeSH Term Description Entries
D008297 Male Males
D001757 Blastomeres Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells. Blastocytes,Blastocyte,Blastomere
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012732 Sex Determination Analysis Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests. Sex Determination Techniques,Sex Determination Technics,Analyses, Sex Determination,Analysis, Sex Determination,Sex Determination Analyses,Sex Determination Technic,Sex Determination Technique,Technic, Sex Determination,Technics, Sex Determination,Technique, Sex Determination,Techniques, Sex Determination
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D018797 Cell Cycle Proteins Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. Cell Division Cycle Proteins,Cell-Cycle Regulatory Proteins,cdc Proteins,Cell Cycle Regulatory Proteins

Related Publications

M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
January 1993, Genetic analysis, techniques and applications,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
February 1994, Science in China. Series B, Chemistry, life sciences & earth sciences,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
March 1994, Journal of the Formosan Medical Association = Taiwan yi zhi,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
September 2004, Theriogenology,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
September 1993, Genomics,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
November 1999, Molecular reproduction and development,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
April 1994, Human reproduction (Oxford, England),
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
May 2012, Theriogenology,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
July 1997, Mammalian genome : official journal of the International Mammalian Genome Society,
M C A Carneiro, and P L Takeuchi, and A Araújo, and R B Lôbo, and F P Elias, and R A Vila, and C L Miranda-Furtado, and E S Ramos
November 2004, Theriogenology,
Copied contents to your clipboard!